SirT1 knockdown potentiates radiation-induced bystander effect through promoting c-Myc activity and thus facilitating ROS accumulation

Mutation Research
Yuexia XieChunlin Shao

Abstract

Radiation-induced bystander effect (RIBE) has important implications for secondary cancer risk assessment during cancer radiotherapy, but the bystander signaling processes, especially under hypoxic condition, are still largely unclear. The present study found that micronuclei (MN) formation could be induced in the non-irradiated HL-7702 hepatocyte cells after being treated with the conditioned medium from irradiated hepatoma HepG2 and SK-Hep-1 cells under either normoxia or hypoxia. This bystander response was dramatically diminished or enhanced when the SirT1 gene of irradiated hepatoma cells was overexpressed or knocked down, respectively, especially under hypoxia. Meanwhile, SirT1 knockdown promoted transcriptional activity for c-Myc and facilitated ROS accumulation. But both of the increased bystander responses and ROS generation due to SirT1-knockdown were almost completely suppressed by c-Myc interference. Moreover, ROS scavenger effectively abolished the RIBE triggered by irradiated hepatoma cells even with SirT1 depletion. These findings provide new insights that SirT1 has a profound role in regulating RIBE where a c-Myc-dependent release of ROS may be involved.

References

Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·I EmeritJ Freitas
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·N S ChandelP T Schumacker
Aug 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chunlin ShaoSeiichi Wada
Sep 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chunlin ShaoKevin M Prise
Mar 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chunlin ShaoKevin M Prise
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hongning ZhouTom K Hei
Mar 6, 2007·Experimental & Molecular Medicine·Jaemin JeongKee-Ho Lee
Jul 7, 2007·Molecular Cell·Zhigang YuanEdward Seto
Jul 13, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yuning SunShengqi Wang
Aug 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mariateresa MancusoAnna Saran
Sep 2, 2008·Cancer Research·Susanne Burdak-RothkammKevin M Prise
Nov 20, 2008·BMB Reports·Eun-Joo Kim, Soo-Jong Um
Apr 15, 2009·The Journal of Cell Biology·Jian YuanZhenkun Lou
Jul 31, 2010·Molecular Cell·Wei Fan, Jianyuan Luo
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mei MingYu-Ying He
Aug 3, 2011·The International Journal of Biochemistry & Cell Biology·Beibei MaoChih-Chuan Liang
Sep 15, 2011·The EMBO Journal·Kim Steen JensenPeter Staller
Dec 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Antje MenssenHeiko Hermeking
Mar 25, 2014·Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis·Mingyuan HeChunlin Shao

❮ Previous
Next ❯

Citations

Jan 18, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Wenzhi TuChunlin Shao
Oct 30, 2016·Acta Biochimica Et Biophysica Sinica·Jie FuHaifeng Song
Apr 6, 2018·PloS One·Kishore Kumar JellaFiona M Lyng
Oct 12, 2017·Radiation Research·Hannah F ThompsonKevin M Prise
Mar 19, 2019·Oxidative Medicine and Cellular Longevity·Shashank ShrishrimalRebecca E Oberley-Deegan
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Jianghong ZhangKevin M Prise

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Ataxia telangiectasia

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis