SIRT1 overexpression in skeletal muscle in vivo induces increased insulin sensitivity and enhanced complex I but not complex II-V functions in individual subsarcolemmal and intermyofibrillar mitochondria

Journal of Physiology and Biochemistry
Hao-Hao ZhangJing Wu

Abstract

SIRT1 is known to improve insulin resistance (IR), but whether this effect is direct or not is still unclear, and this question has not been addressed in vivo in the skeletal muscle. Therefore, we sought to test if acute overexpression of SIRT1 in skeletal muscle of high-fat diet (HFD) rats in vivo would affect subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondrial complexes I-V activities and antioxidant enzymes thereby improving insulin action. In vivo electrotransfer was used to overexpress SIRT1 in the skeletal muscle of rats fed HFD for 12 weeks. Skeletal muscle insulin sensitivity and downstream effects of SIRT1 on AMPK, SIRT3, and mitochondrial biogenesis were studied. Citrate synthase (CS), complexes I-V, oxidative stress, and antioxidant levels were assessed in SS and IMF mitochondria. HFD rats showed skeletal muscle IR as well as decreased SIRT1 and SIRT3 expressions, mitochondrial DNA (mtDNA), and mitochondrial biogenesis (p < 0.05). SS and IMF mitochondria displayed lower CS, complexes I-V, and antioxidant enzyme activities (p < 0.05). By contrast, moderate (~2.5 folds) SIRT1 overexpression attenuated HFD-induced skeletal muscle IR. This improvement was associated with increased AMPK, PGC-1α, SIRT3, and mtDNA...Continue Reading

References

May 29, 2004·American Journal of Physiology. Endocrinology and Metabolism·Luc J C van LoonHans A Keizer
Nov 29, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jonathan D SchertzerGordon S Lynch
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Biplab Dasgupta, Jeffrey Milbrandt
Apr 21, 2007·Circulation Research·Ralph R AlcendorJunichi Sadoshima
Jul 20, 2007·International Journal of Obesity : Journal of the International Association for the Study of Obesity·L LionettiS Iossa
Nov 28, 2007·The Journal of Biological Chemistry·Helena M Cochemé, Michael P Murphy
Jan 29, 2008·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Gaffari TürkHakan Aksoy
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Jan 15, 2009·American Journal of Physiology. Endocrinology and Metabolism·Graham P HollowayArend Bonen
May 7, 2009·Cell Metabolism·Bei B ZhangCai Li
Dec 24, 2009·American Journal of Physiology. Endocrinology and Metabolism·Joachim NielsenNiels Ortenblad
May 29, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Michael J RyanStephen E Alway
Sep 9, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Jean-Louis BeaudeuxPhilippe Giral
Feb 16, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yu LiMengwei Zang
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Enxuan JingC Ronald Kahn
Jun 26, 2012·Trends in Endocrinology and Metabolism : TEM·Joris Hoeks, Patrick Schrauwen
Sep 13, 2012·American Journal of Physiology. Endocrinology and Metabolism·Hongliang LiZhonglin Xie

❮ Previous
Next ❯

Citations

Oct 1, 2015·Journal of Diabetes and Its Complications·Yue CaoYan Liu
Oct 27, 2017·Archives of Physiology and Biochemistry·Abhijit Nirwane, Anuradha Majumdar
Nov 25, 2016·Neurochemical Research·Henryk JęśkoJoanna B Strosznajder
Apr 15, 2017·Journal of Cellular Biochemistry·Hao-Hao ZhangYan-Hui Bai
Aug 6, 2017·Biochimie·Alicia Mayeuf-LouchartHélène Duez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.