SIRT1 suppresses doxorubicin-induced cardiotoxicity by regulating the oxidative stress and p38MAPK pathways

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Yang RuanJian Li

Abstract

SIRT1, which belongs to the Sirtuin family of NAD-dependent enzymes, plays diverse roles in aging, metabolism, and disease biology. It could regulate cell survival and has been shown to be a protective factor in heart function. Hence, we verified the mechanism by which SIRT1 regulates doxorubicin induced cardiomyocyte injury in vivo and in vitro. We analyzed SIRT1 expression in doxorubicin-induced neonatal rat cardiomyocyte injury model and adult mouse heart failure model. SIRT1 was over-expressed in cultured neonatal rat cardiomyocyte by adenovirus mediated gene transfer. SIRT1 agonist resveratrol was used to treat the doxorubicin-induced heart failure mouse model. Echocardiography, reactive oxygen species (ROS) production, TUNEL, qRT-PCR, and Western blotting were performed to analyze cell survival, oxidative stress, and inflammatory signal pathways in cardiomyocytes. SIRT1 expression was down-regulated in doxorubicin induced cardiomocyte injury, accompanied by elevated oxidative stress and cell apoptosis. SIRT1 over-expression reduced doxorubicin induced cardiomyocyte apoptosis with the attenuated ROS production. SIRT1 also reduced cell apoptosis by inhibition of p38MAPK phosphorylation and caspase-3 activation. The SIRT1 ag...Continue Reading

Citations

Jun 24, 2015·Age·Gaia FaveroRita Rezzani
Mar 24, 2017·British Journal of Pharmacology·Thomas KietzmannAgnes Görlach
Nov 7, 2017·Nutrients·Guilherme Felipe Santos FernandesJean Leandro Dos Santos
Aug 31, 2017·Bulletin of Experimental Biology and Medicine·K L KozlovA O Drobintseva
Sep 13, 2020·Journal of Cellular and Molecular Medicine·Wei-Yin WuYan Wang
Feb 9, 2020·Cardiovascular Drugs and Therapy·Kerstin N Timm, Damian J Tyler
Nov 9, 2017·Molecular Medicine Reports·Fengmei TanChan Zhang
Dec 14, 2017·Oxidative Medicine and Cellular Longevity·Niria Treviño-Saldaña, Gerardo García-Rivas
May 24, 2018·Environmental Science and Pollution Research International·Na ChenYa-Min Li
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Yan-Zhao WuChen Xiong
Jul 18, 2020·Clinical Science·Keith DadsonFilio Billia
Jan 14, 2021·Cellular and Molecular Life Sciences : CMLS·Jie Wang AJunlian Gu
Sep 24, 2019·Chemico-biological Interactions·Alina HanfAndreas Daiber
Feb 7, 2021·Basic & Clinical Pharmacology & Toxicology·Ali A Shati, Attalla F El-Kott
Nov 14, 2020·Clinical Science·Alexa N CarreraBeshay N Zordoky
Apr 3, 2021·Cell Death & Disease·Effimia Christidi, Liam R Brunham
Oct 28, 2019·The Journal of Thoracic and Cardiovascular Surgery·Raphael Dos Santos Coutinho E SilvaLuiz Felipe Pinho Moreira
Apr 12, 2021·Trends in Molecular Medicine·Xavier PalomerManuel Vázquez-Carrera
Aug 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Irshad Ahmad, Muddasarul Hoda
Aug 8, 2021·Redox Biology·Daisong LiXianming Chu
Nov 9, 2021·Journal of Biochemical and Molecular Toxicology·Fatemeh B TabriziGholamreza Karimi

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