Sirt3-MnSOD axis represses nicotine-induced mitochondrial oxidative stress and mtDNA damage in osteoblasts

Acta Biochimica Et Biophysica Sinica
Yong LiJianping Gong

Abstract

Increasing evidence has suggested an important role played by reactive oxygen species in the pathogenesis of osteoporosis. Tobacco smoking is an important risk factor for the development of osteoporosis, and nicotine is one of the major components in tobacco. However, the mechanism by which nicotine promotes osteoporosis is not fully understood. Here, in this study, we found that nicotine-induced mitochondrial oxidative stress and mitochondrial DNA (mtDNA) damage in osteoblasts differentiated from mouse mesenchymal stem cell. The activity of MnSOD, one of the mitochondrial anti-oxidative enzymes, was significantly reduced by nicotine due to the reduced level of Sirt3. Moreover, it was also found that Sirt3 could promote MnSOD activity by deacetylating MnSOD. Finally, Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP, a MnSOD mimetic) was found to markedly reduce the effect of nicotine on osteoblasts. In summary, Sirt3-MnSOD axis was identified as a negative component in nicotine-induced mitochondrial oxidative stress and mtDNA damage, and MnTBAP may serve as a potential therapeutic drug for osteoporosis.

References

Mar 18, 2008·Free Radical Biology & Medicine·Jennifer E BruinAlison C Holloway
May 13, 2011·Clinical Oral Implants Research·Li MaMai Har Sham
Aug 11, 2011·The Journal of Obstetrics and Gynaecology Research·Vikram Sinai TalaulikarIsaac Manyonda
Aug 31, 2011·Molecular and Cellular Biology·Wei WeiYihong Wan
Sep 12, 2012·Experimental Gerontology·Alexandra S Bause, Marcia C Haigis
Oct 24, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Carlo CervellatiLeo Massari
Feb 1, 2013·EMBO Molecular Medicine·Petra SimicLeonard Guarente
Feb 16, 2013·Expert Opinion on Drug Safety·Rami KallalaPeter V Giannoudis
Mar 5, 2013·Experimental Gerontology·Chul Han, Shinichi Someya
Apr 27, 2013·The FEBS Journal·Christian DölleMathias Ziegler
Aug 29, 2013·The Biochemical Journal·Alberto BartoloméCarlos Guillén
Nov 12, 2013·Biochemical and Biophysical Research Communications·Jiangtao LinYanping Zheng
Mar 19, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Anna EnglerCaroline Ospelt
May 17, 2014·Frontiers in Physiology·Xiaoqiang TangDe-Pei Liu

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Citations

Dec 30, 2016·Arhiv za higijenu rada i toksikologiju·Milica G PaunovićZorica S Saičić
Jul 8, 2017·Circulation Research·Anna E DikalovaSergey I Dikalov
Jan 9, 2019·Antioxidants & Redox Signaling·Sergey I Dikalov, Anna E Dikalova
Jan 5, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Sergey DikalovAnna Dikalova
Dec 31, 2015·Current Opinion in Nephrology and Hypertension·Sergey I Dikalov, Anna E Dikalova
Sep 8, 2019·Journal of Bone and Mineral Metabolism·Xiangchang CaoDasheng Lin
Dec 15, 2015·Journal of Applied Physiology·Chun LiWilliam G Mayhan
Feb 23, 2021·Journal of Neurochemistry·Dennison TrinhJoanne E Nash
May 7, 2021·Journal of Inflammation·Thomas NicholsonSimon W Jones

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