Sirt3 prevents maternal obesity-associated oxidative stress and meiotic defects in mouse oocytes

Cell Cycle
Liang ZhangQiang Wang

Abstract

Maternal obese environment has been reported to induce oxidative stress and meiotic defects in oocytes, however the underlying molecular mechanism remains unclear. Here, using mice fed a high fat diet (HFD) as an obesity model, we first detected enhanced reactive oxygen species (ROS) content and reduced Sirt3 expression in HFD oocytes. We further observed that specific depletion of Sirt3 in control oocytes elevates ROS levels while Sirt3 overexpression attenuates ROS production in HFD oocytes, with significant suppression of spindle disorganization and chromosome misalignment phenotypes that have been reported in the obesity model. Candidate screening revealed that the acetylation status of lysine 68 on superoxide dismutase (SOD2K68) is dependent on Sirt3 deacetylase activity in oocytes, and acetylation-mimetic mutant SOD2K68Q results in almost threefold increase in intracellular ROS. Moreover, we found that acetylation levels of SOD2K68 are increased by ~80% in HFD oocytes and importantly, that the non-acetylatable-mimetic mutant SOD2K68R is capable of partially rescuing their deficient phenotypes. Together, our data identify Sirt3 as an important player in modulating ROS homeostasis during oocyte development, and indicate tha...Continue Reading

References

Apr 3, 2001·Nature Reviews. Genetics·T Hassold, P Hunt
Mar 27, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·Alexandra J HarveyJeremy G Thompson
Sep 18, 2004·Fertility and Sterility·Mohamed A BedaiwyAshok Agarwal
Mar 1, 2005·Cell·Robert S BalabanToren Finkel
Jun 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bjoern SchwerEric Verdin
Nov 11, 2006·Human Reproduction·D C Gesink LawM P Longnecker
Oct 10, 2007·Molecular and Cellular Biology·David B LombardBjoern Schwer
Dec 13, 2007·American Journal of Physiology. Endocrinology and Metabolism·Sarah L WakefieldMegan Mitchell
Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Bong-Hyun AhnToren Finkel
May 6, 2009·Human Reproduction Update·João Ramalho-SantosAlexandra Amaral
Jun 17, 2009·Seminars in Cell & Developmental Biology·R DumollardK Swann
Aug 27, 2010·American Journal of Obstetrics and Gynecology·Emily S Jungheim, Kelle H Moley
Nov 13, 2010·Human Reproduction·Barbara LukeUNKNOWN SART Writing Group
Nov 27, 2010·Cell Metabolism·Xiaolei QiuDanica Chen
Jun 15, 2011·Free Radical Biology & Medicine·Tetsuro KoyamaTakashi Uzu
Apr 27, 2012·The Biochemical Journal·Albert Giralt, Francesc Villarroya
Jul 25, 2012·The Journal of Cell Biology·Yue Xiong, Kun-Liang Guan
Sep 12, 2012·Experimental Gerontology·Alexandra S Bause, Marcia C Haigis
Sep 13, 2012·Human Reproduction·Ronit MachtingerCatherine Racowsky
Dec 5, 2012·Cold Spring Harbor Perspectives in Biology·Michael N Sack, Toren Finkel
Dec 25, 2012·Free Radical Biology & Medicine·Faten ShaeibHusam M Abu-Soud
Feb 5, 2013·Cell Reports·Katharine BrownDanica Chen
Apr 25, 2013·Molecular Human Reproduction·Natalia M Grindler, Kelle H Moley
May 15, 2013·Free Radical Biology & Medicine·Anne H H TsengDanny Ling Wang
Dec 18, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Liang ZhangQiang Wang
Jun 26, 2014·Human Reproduction·Giovanna Di EmidioCarla Tatone

❮ Previous
Next ❯

Citations

Jan 29, 2016·Science Translational Medicine·Kelle H Moley, Graham A Colditz
Jun 24, 2015·Age·Gaia FaveroRita Rezzani
May 20, 2018·Molecular & Cellular Proteomics : MCP·Haichao WangQiang Wang
Jul 8, 2017·Circulation Research·Anna E DikalovaSergey I Dikalov
Nov 28, 2018·Journal of Cellular Physiology·Xiaoyan LiLing Gu
Mar 3, 2018·Pediatric Cardiology·Alessandro IanniThomas Braun
Dec 17, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Long T NguyenSonia Saad
Jun 8, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Min GaoLing Gu
Jan 28, 2019·Science China. Life Sciences·Kexin ZouHefeng Huang
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Samprikta MannaFergus P McCarthy
Mar 27, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juvita D Iljas, Hayden A Homer
May 5, 2017·Scientific Reports·Li-Feng LiangWei-Hua Wang
Dec 12, 2018·Journal of Cellular Physiology·Xiang-Hong OuShao-Chen Sun
Aug 19, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Natalia Llarena, Christopher Hine
Sep 12, 2020·Aging·Huafang WeiWei Li
Sep 22, 2020·American Journal of Physiology. Endocrinology and Metabolism·Lucy Anne BarthoAnthony V Perkins
Nov 13, 2020·Cell Death & Disease·Yongzhi CaoYueran Zhao
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Xin WenCheng-Guang Liang
Nov 14, 2020·Molecular Reproduction and Development·Berta N VazquezKaren Schindler
Oct 20, 2020·The Journal of Reproduction and Development·Ryutaro AizawaSatoshi Tsukamoto
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Meina HeChao Wang
Jul 28, 2021·Current Biology : CB·Emmanouella E ChatzidakiKikuë Tachibana
Sep 1, 2021·Reproductive Toxicology·Zuzana ŠefčíkováDušan Fabian

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
in vitro transcription
confocal microscopy

Software Mentioned

ImageJ
Image J
Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.