Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway

Molecular Medicine Reports
Zhi-Gao Zhang, Cheng-Yong Qin

Abstract

Sirt6, a member of the mammalian sirtuin family, is a protein that is located in the nucleus and is an NAD+‑dependent deacetylase important in the control of metabolic activity and genome stability. Recently, several studies have demonstrated the potential role of Sirt6 in tumor biology; however, the role of Sirt6 in hepatocellular carcinoma (HCC) remains unclear. In the present study, Sirt6 protein expression was found to be downregulated in human HCC tissue compared with adjacent normal tissue. Knockdown of Sirt6 promoted growth of the HepG2 HCC cell line, whereas overexpression of Sirt6 inhibited the growth of HepG2 cells. Overexpression of Sirt6 induced apoptosis in HepG2 cells, which was demonstrated by a terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay and cleaved caspase-3 immunoblotting. Furthermore, overexpression of Sirt6 decreased intracellular reactive oxygen species and superoxide anion levels. Finally, overexpression of Sirt6 inhibited phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2), and blocking the ERK1/2 pathway with chemical-specific inhibitor U0126, attenuated the tumor suppressive effect of overexpression of Sirt6. Collectively, these data suggest that Sirt6 ...Continue Reading

References

Mar 3, 2004·Journal of the American College of Surgeons·Chad A WiesenauerC Max Schmidt
Oct 28, 2004·Gastroenterology·F Xavier BoschRamon Cléries
Aug 2, 2006·EMBO Reports·Satoko Nishimoto, Eisuke Nishida
Nov 3, 2006·Genes & Development·Marcia C Haigis, Leonard P Guarente
May 10, 2007·Nature Reviews. Microbiology·Darius MoradpourCharles M Rice
Jun 16, 2007·Gastroenterology·Hashem B El-Serag, K Lenhard Rudolph
Feb 27, 2009·Cancer Research·Tao LiuGlenn M Marshall
Jul 16, 2010·Nature Reviews. Gastroenterology & Hepatology·Ju Dong Yang, Lewis R Roberts
Sep 11, 2010·Science·Abderrahmane KaidiStephen P Jackson
Jan 6, 2011·International Journal of Cancer. Journal International Du Cancer·Eva ColasAntonio Gil-Moreno
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Mar 15, 2011·Cancer Cell·Lydia W S FinleyMarcia C Haigis
Apr 8, 2011·Cancer·Turki Y AlhazzaziYvonne L Kapila
May 19, 2011·Biochimica Et Biophysica Acta·Turki Y AlhazzaziYvonne L Kapila
Jun 18, 2011·Science·Zhiyong MaoVera Gorbunova
May 23, 2012·Biochemical and Biophysical Research Communications·Yuan-Yuan Zhang, Li-Ming Zhou
Mar 12, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chi-Chih LaiMing-Yu Yang
Mar 22, 2013·Carcinogenesis·Mattaka KhongkowEric W-F Lam

❮ Previous
Next ❯

Citations

Jul 18, 2015·FEBS Letters·Tomohiko FukudaTomoyuki Fujii
Oct 2, 2015·Cells·Mareike BütepageBernhard Lüscher
Jun 22, 2017·Marine Drugs·Minna K Rahnasto-RillaRuin Moaddel
Apr 27, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Peng-Chen DaiGuo-Xiang Lai
Dec 15, 2015·Rejuvenation Research·Xiao-Yan ZhaiRen-Ke Li
Jun 30, 2019·Cell Cycle·Luis Filipe Costa-Machado, Pablo J Fernandez-Marcos
Jun 29, 2017·Oncology Reports·Jianmei ZhouHui Dai
Oct 9, 2019·International Journal of Molecular Sciences·Maria de Céu TeixeiraEliana B Souto
May 26, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sun-O KaByung-Hyun Park
Jun 11, 2020·The Journal of Biological Chemistry·Mark A Klein, John M Denu
Jan 1, 2016·Carcinogenesis·Batia LerrerHaim Y Cohen
Nov 27, 2015·Chembiochem : a European Journal of Chemical Biology·Minna Rahnasto-RillaRuin Moaddel
Oct 22, 2016·Pathology Oncology Research : POR·Zsuzsanna GaálLászló Csernoch
Feb 23, 2019·Expert Review of Gastroenterology & Hepatology·Jun ZhaoMatthew W Lawless
Jul 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Berin Karaman MayackFidele Ntie-Kang
Feb 15, 2019·Frontiers in Pharmacology·Vincenzo CarafaAngela Nebbioso
Nov 18, 2020·Journal of Experimental & Clinical Cancer Research : CR·Zhongkai ZhangKyu Yun Jang
Dec 17, 2020·Medicinal Research Reviews·Gang LiuJia Zhou
Jan 13, 2021·Cancers·Aranka BrockmuellerMehdi Shakibaei
Dec 19, 2020·Open Medicine·Li ShiYadong Wang
Apr 4, 2021·Cancers·Francesco FiorentinoDante Rotili
Aug 3, 2014·Disease Models & Mechanisms·Bernadette M M Zwaans, David B Lombard
Jul 3, 2021·Journal of Medicinal Chemistry·Francesco FiorentinoDante Rotili

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