SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation

Journal of Experimental & Clinical Cancer Research : CR
Wenlu LiShuaihua Qin

Abstract

Oral squamous cell carcinoma (OSCC) is one of the most common malignancies and has a poor prognosis. The epithelial-to-mesenchymal transition (EMT) is crucial for increasing the metastasis of OSCC. Recently, studies have indicated that sirtuin7 (SIRT7) is implicated in tumor genesis; however, the potential role of SIRT7 in the EMT and metastasis of OSCC has not been reported. We investigated the cellular responses to SIRT7 silencing or overexpression in OSCC cell lines by wound healing assay, migration and invasion assay, western blotting, immunofluorescence and immunohistochemistry. In the present study, we found that SIRT7 was significantly downregulated in OSCC cell lines and human OSCC/OSCC tissues with lymph node metastasis. Overexpression of SIRT7 decreased the proliferation and invasion of OSCC cells in vitro, whereas SIRT7 knockdown significantly increased OSCC cell growth and invasion. Upregulation of SIRT7 concomitantly increased the expression of E-cadherin, and decreased the expression of mesenchymal markers. SIRT7 overexpression also reduced the level of acetylated SMAD4 in OSCC cells. Moreover, SIRT7 overexpression significantly inhibited OSCC lung metastasis in vivo. Together, these findings suggested that SIRT7 ...Continue Reading

References

Jun 23, 2012·Nature·Matthew F BarberKatrin F Chua
Mar 12, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chi-Chih LaiMing-Yu Yang
Apr 29, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hongyan YuWenlin Huang
Dec 19, 2014·BMC Cancer·Chirayu Pankaj Goswami, Harikrishna Nakshatri
Apr 30, 2015·Scientific Reports·Shivani MalikKatrin F Chua
Jul 6, 2016·Oxidative Medicine and Cellular Longevity·Raffaele PalmirottaFranco Silvestris
Aug 9, 2016·Journal of Pathology and Translational Medicine·Hye Seung LeeBaek-Hui Kim
Feb 18, 2017·Cancer Research·Tomohiro TamuraKazuhiro Morishita
May 10, 2017·Journal of Oral and Maxillofacial Pathology : JOMFP·Dhanu G RaoS P Shrutha
Jul 13, 2017·Virchows Archiv : an International Journal of Pathology·Nina ZidarVojko Didanovič
Aug 23, 2017·Nature Communications·Xiaolong TangBaohua Liu
Sep 26, 2017·Molecular Medicine Reports·Di Li, Lifei Li
Nov 28, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hao WangShaofen Huo
Dec 7, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Man Ki ChungJohn B Sunwoo
Feb 11, 2018·Biochemical and Biophysical Research Communications·Tian XieChongjun Zhong
Feb 14, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenzhi LiJie Tao

❮ Previous
Next ❯

Citations

Nov 7, 2019·Cancer Biomarkers : Section a of Disease Markers·Chaonan SunNa Zhang
Mar 16, 2019·Cellular & Molecular Biology Letters·Bingxia ShiYanjun Guo
Aug 9, 2020·Oral Diseases·Sabrina MarcazzanMauro Ferrari
Feb 6, 2020·Journal of Experimental & Clinical Cancer Research : CR·Mao DingShu-Jie Xia
Feb 15, 2019·Frontiers in Pharmacology·Vincenzo CarafaAngela Nebbioso
Dec 11, 2019·Cancers·Erhu ZhaoHongjuan Cui
Jul 14, 2020·Frontiers in Oncology·Shashi KiranGayatri Ramakrishna
Jan 13, 2021·Cancers·Aranka BrockmuellerMehdi Shakibaei
Feb 12, 2021·Oral Diseases·Devaraj EzhilarasanSubramanian Raghunandhakumar
Jun 3, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alberto ZulloWerner Klingler

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
Assay
xenograft

Software Mentioned

Image J

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.