Sirtuin-1 Controls Poly (I:C)-Dependent Matrix Metalloproteinase 9 Activation in Primary Human Nasal Epithelial Cells

American Journal of Respiratory Cell and Molecular Biology
Masanobu SuzukiSarah Vreugde

Abstract

Matrix metalloproteinase (MMP)-9 is thought to be involved in the etiopathogenesis of chronic rhinosinusitis (CRS) with nasal polyps and cleaves collagen IV, causing hyperpermeability of the basement membrane within mucosal tissue. It is known that MMP-9 expression is negatively affected by sirtuin (SIRT)-1 in human monocytotic cells, retinal endothelial cells, and epithelial carcinoma cells. However, it is unknown which factors affect MMP-9 expression and activity in human nasal epithelial cells (HNECs). To examine factors affecting MMP-9 expression and activity in HNECs, HNECs were stimulated with Toll-like receptor (TLR) agonists, followed by quantitative PCR, immunofluorescence, and zymography to examine MMP-9 expression and activity. MMP-9 expression was evaluated in sinonasal tissue of control subjects without CRS, and patients with CRS without nasal polyps and those with CRS with nasal polyps, in relation to the expression of SIRT1 using a tissue microarray. The effect of SIRT1 stimulation/inhibition on MMP-9 expression in HNECs was also tested. TLR3 agonists increased MMP-9 mRNA expression (473 fold, P = 0.0198) and activity (20.4-fold, P < 0.05). SIRT1 activation or inhibition reciprocally affected MMP-9 expression in ...Continue Reading

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Citations

Jul 12, 2018·American Journal of Respiratory Cell and Molecular Biology·Jonathan R Baker
Apr 16, 2020·Frontiers in Cell and Developmental Biology·Anu Laulajainen-HongistoRobert Kern
Aug 23, 2020·Journal of Clinical Medicine·Yosuke FukudaHironori Sagara
Feb 3, 2021·Auris, Nasus, Larynx·Masanobu SuzukiSarah Vreugde
Nov 1, 2020·Allergology International : Official Journal of the Japanese Society of Allergology·Masanobu SuzukiAkihiro Homma
May 14, 2021·Frontiers in Cellular and Infection Microbiology·Akira NakazonoAkihiro Homma

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Methods Mentioned

BETA
PCR
zymography
ELISA
nuclear translocation

Software Mentioned

JMP

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