Sirtuin 1 regulates hepatitis B virus transcription and replication by targeting transcription factor AP-1

Journal of Virology
Ji-Hua RenJuan Chen

Abstract

Chronic hepatitis B virus (HBV) infection is a major risk factor for liver cirrhosis and hepatocellular carcinoma. Nevertheless, the molecular mechanism of HBV replication remains elusive. SIRT1 is a class III histone deacetylase that is a structure component of the HBV cccDNA minichromosome. In this study, we found by using microarray-based gene expression profiling analysis that SIRT1 was upregulated in HBV-expressing cells. Gene silencing of SIRT1 significantly inhibited HBV DNA replicative intermediates, 3.5-kb mRNA, and core protein levels. In contrast, the overexpression of SIRT1 augmented HBV replication. Furthermore, SIRT1 enhanced the activity of HBV core promoter by targeting transcription factor AP-1. The c-Jun subunit of AP-1 was bound to the HBV core promoter region, as demonstrated by using a chromatin immunoprecipitation assay. Mutation of AP-1 binding site or knockdown of AP-1 abolished the effect of SIRT1 on HBV replication. Finally, SIRT1 inhibitor sirtinol also suppressed the HBV DNA replicative intermediate, as well as 3.5-kb mRNA. Our study identified a novel host factor, SIRT1, which may facilitate HBV replication in hepatocytes. These data suggest a rationale for the use of SIRT1 inhibitor in the treatmen...Continue Reading

References

Oct 24, 2001·Cell·H VaziriR A Weinberg
May 4, 2002·Nature Cell Biology·Eitan Shaulian, Michael Karin
Sep 4, 2004·Seminars in Liver Disease·Nezam H Afdhal
Feb 19, 2005·PLoS Biology·Sara PagansMelanie Ott
Jul 25, 2006·Critical Reviews in Oncology/hematology·Alejandro FornerJordi Bruix
Aug 8, 2006·Nature Cell Biology·Chuangui WangJiandong Chen
Apr 17, 2007·Cell Biology International·Chang-Su Lim
Apr 24, 2007·The Biochemical Journal·Shaday Michan, David Sinclair
Jan 10, 2009·Nature Reviews. Cancer·Christopher L Brooks, Wei Gu
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laura BelloniMassimo Levrero
Jan 1, 2010·The Journal of Biological Chemistry·Ran ZhangChih-Chuan Liang
Jan 19, 2010·Annual Review of Pathology·Marcia C Haigis, David A Sinclair
Jun 16, 2010·Journal of Viral Hepatitis·M Quasdorff, U Protzer
Oct 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Maria F PerdomoAnders Vahlne

❮ Previous
Next ❯

Citations

Aug 4, 2015·Journal of Clinical Biochemistry and Nutrition·Mona S Ottum, Anahita M Mistry
Jan 1, 2016·Cellular and Molecular Life Sciences : CMLS·Inês MesquitaRicardo Silvestre
Sep 11, 2014·Pharmacology & Therapeutics·Ioannis AnestopoulosMihalis I Panayiotidis
Dec 9, 2014·Cell Cycle·Meilan He, Shou-Jiang Gao
Oct 20, 2015·Reviews in Medical Virology·Tao YangJianzhong Cao
Sep 22, 2015·Biochemical and Biophysical Research Communications·Tatsuo KandaOsamu Yokosuka
Jun 10, 2014·The International Journal of Biochemistry & Cell Biology·Shih-Che HsuYu-Juei Hsu
Dec 18, 2014·MBio·Emre KoyuncuIleana M Cristea
Apr 22, 2016·Scientific Reports·Yixian ShiZhenggang Yang
Jul 6, 2017·Critical Reviews in Food Science and Nutrition·Shubhra PandeAlla B Salmina
Jun 21, 2018·Journal of Cellular Biochemistry·AmirReza HesariHamed Mirzaei
Jul 11, 2019·MBio·Brent A HackettSara Cherry
Sep 5, 2019·International Journal of Molecular Sciences·Nur K Mohd-IsmailYee-Joo Tan
Aug 1, 2019·Current Drug Targets·Bing-Yi ZhangXiao-Ping Yu
Sep 5, 2019·Viral Immunology·Asghar AbdoliAmitis Ramezani
Jun 29, 2016·World Journal of Gastroenterology : WJG·Hong KimBum-Joon Kim
Nov 20, 2019·The Journal of Infectious Diseases·Ashwin BalagopalChloe L Thio
Sep 16, 2016·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Charles L Raison, Andrew H Miller
Jul 11, 2020·Infectious Agents and Cancer·Ying YangZhi Chen
Dec 4, 2019·Reviews in Medical Virology·Habibollah MirzaeiSayyad Khanizadeh
Jun 3, 2021·Pharmaceuticals·Georgia-Myrto PriftiGrigoris Zoidis
Jun 25, 2021·World Journal of Gastroenterology : WJG·Prashika SinghKristie Bloom

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.