Abstract
Sirtuin 7 (Sirt7) is a member of the sirtuin protein family and is implicated in various carcinomas; however, the function of Sirt7 in colorectal carcinoma (CRC) remains unclear. The present study aimed to explore the biological function of Sirt7 in CRC tissues and cell lines, and to investigate the potential underlying mechanism by performing reverse transcription-quantitative polymerase chain reaction analyses, western blot analyses, luciferase reporter assays, cell proliferation and invasion assays. It was demonstrated that Sirt7 presented a higher expression in CRC tissues and cell lines compared with that in normal tissues and cells, and this higher expression was correlated with the tumor size, the tumor, node and metastasis stage and distant metastasis. Knockdown of Sirt7 repressed the proliferation ability of SW620 and HCT116 cellsin vitro, while ectopic expression of Sirt7 increased the epithelial-mesenchymal transition and invasion in HT29 and SW480 cells. Notably, these functional effects of Sirt7 were exerted through the repression of E-cadherin. Thus, the data of the present study indicated a novel mechanistic role for Sirt7 as an oncogene in CRC malignancy, and Sirt7 may be a potential therapeutic target.
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 7, 2004·Journal of the National Cancer Institute·Jessica B O'ConnellClifford Y Ko
Jul 13, 2005·Gut·N Andre, W Schmiegel
Sep 28, 2006·British Journal of Cancer·N AshrafP G Shiels
Jun 11, 2009·Molecular Cancer Therapeutics·Leigh EllisRicky W Johnstone
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Aug 23, 2011·Clinical Colorectal Cancer·Renata FerrarottoScott Kopetz
Dec 22, 2011·Biochimica Et Biophysica Acta·V CalabreseP Castellino
Jan 18, 2012·Oncogene·V BylesY Dai
Jun 23, 2012·Nature·Matthew F BarberKatrin F Chua
Oct 2, 2012·Diabetes Management·X Charlie Dong
Oct 20, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jeong Kyu KimSuk Woo Nam
Jul 17, 2013·Biochemical Pharmacology·Li ZhangJin-Ming Yang
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Feb 5, 2014·Cancer Letters·Sarah KrausNadir Arber
Feb 12, 2014·FEBS Letters·Yonghua HanZhiming Cai
Mar 4, 2014·World Journal of Gastroenterology : WJG·In Ja Park, Chang Sik Yu
Apr 29, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hongyan YuWenlin Huang
May 13, 2014·Medical Oncology·Liang LvShan Wang
Aug 31, 2014·Cancer Letters·Shichang DengGang Zhao
Dec 17, 2014·Scientific Reports·Xiaojing ChenLixin Wei
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Mar 22, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sapna SinghGayatri Ramakrishna
Apr 11, 2015·Scientific Reports·Shun ZhangTing Cai
Apr 30, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Hong-Ling WangLin Guo
Dec 2, 2015·Experimental and Therapeutic Medicine·Mengyao JiXiulan Peng
Dec 2, 2015·Oncology Letters·Yifeng ZouPing Lan
Mar 16, 2016·Cancer Letters·Feifei ChengCheng Qian
Aug 31, 2016·Redox Report : Communications in Free Radical Research·Suhee KimTae-Hoon Lee