Sister chromatid cohesion role for CDC28-CDK in Saccharomyces cerevisiae.

Genetics
Alex Brands, Robert V Skibbens

Abstract

High-fidelity chromosome segregation requires that the sister chromatids produced during S phase also become paired during S phase. Ctf7p (Eco1p) is required to establish sister chromatid pairing specifically during DNA replication. However, Ctf7p also becomes active during G2/M in response to DNA damage. Ctf7p is a phosphoprotein and an in vitro target of Cdc28p cyclin-dependent kinase (CDK), suggesting one possible mechanism for regulating the essential function of Ctf7p. Here, we report a novel synthetic lethal interaction between ctf7 and cdc28. However, neither elevated CDC28 levels nor CDC28 Cak1p-bypass alleles rescue ctf7 cell phenotypes. Moreover, cells expressing Ctf7p mutated at all full- and partial-consensus CDK-phosphorylation sites exhibit robust cell growth. These and other results reveal that Ctf7p regulation is more complicated than previously envisioned and suggest that CDK acts in sister chromatid cohesion parallel to Ctf7p reactions.

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J E Kranz, C Holm
Jan 1, 1987·Methods in Enzymology·J D BoekeG R Fink
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Aug 23, 1996·Cell·P KaldisM J Solomon
Aug 23, 1996·Cell·J Y ThuretC Mann
Dec 1, 1996·Molecular and Cellular Biology·C F Hardy, A Pautz
Dec 11, 1997·Molecular & General Genetics : MGG·K T Chun, M G Goebl
Mar 28, 1998·Science·A Dillin, J Rine
Oct 17, 1998·Molecular and Cellular Biology·F H EspinozaD O Morgan
Apr 25, 2000·Molecular and Cellular Biology·K TanakaH Okayama
Nov 22, 2000·The Journal of Cell Biology·S LalorayaD Koshland
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M WeinreichB Stillman
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeanette Gowen CookJoseph R Nevins
Jan 30, 2002·The Journal of Cell Biology·David E AndersonTatsuya Hirano
May 2, 2002·Molecular Cell·Christian H HaeringKim Nasmyth
Sep 7, 2002·Molecular and Cellular Biology·Sheng Yao, Gregory Prelich
Oct 3, 2002·The Journal of Biological Chemistry·Ana A Kitazono, Stephen J Kron
Mar 26, 2003·Cell·Stephan GruberKim Nasmyth
Jun 27, 2003·Molecular Genetics and Genomics : MGG·A A KitazonoS J Kron
Oct 24, 2003·Nature·Jeffrey A UbersaxDavid O Morgan
Jan 27, 2004·Molecular Biology of the Cell·Cheryl D WarrenForrest A Spencer
Jan 27, 2004·Molecular Biology of the Cell·Melanie L MayerPhilip Hieter
Aug 17, 2004·PLoS Biology·Earl F GlynnJennifer L Gerton

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.