Sister chromatid exchanges in ring chromosomes following X-irradiation of human lymphocytes

International Journal of Radiation Biology
R KandaI Hayata

Abstract

To examine whether X-rays induce sister chromatid exchanges (SCE). Peripheral lymphocytes irradiated in vitro or in vivo were cultured and treated with okadaic acid to generate premature chromosome condensation (PCC). When identical spreads were analysed using conventional Giemsa staining and pan-centromeric fluorescence in situ hybridization painting, ring chromosomes were observed. In PCC preparations, cells in the late G(2) phase and late M phase were observed. In late M phase cells, 17-20% of ring chromosomes lacked one chromatid (single-chromatid ring), irrespective of dose. Both the distribution patterns of centromeres in rings and intercentromere distances in dicentric rings indicate that a considerable number of single-chromatid rings might be formed by SCE occurring in a chromosome-type ring, thereby joining strands of two rings, followed by a transformation into one ring. These single-chromatid rings were less stable in vivo than chromosome-type rings. Single-chromatid rings visualized clearly using PCC techniques indicate SCE in the respective rings. Contrary to the conventional SCE-detecting technique, this approach does not require the use of bromodeoxyuridine, which itself leads to SCE. Some of the observed SCE mi...Continue Reading

References

Jan 1, 1980·Human Genetics·A IeshimaY Kuroki
Feb 7, 2001·International Journal of Radiation Biology·R KandaD C Lloyd
Aug 26, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·E BruckmannG Obe
Nov 14, 2002·International Journal of Radiation Biology·R KandaI Hayata

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Citations

Sep 14, 2012·Molecular Medicine·Bess LevinBernard Friedenson
Oct 21, 2004·Analytical Biochemistry·Masahiro MurakamiIsamu Hayata
Jul 28, 2016·International Journal of Radiation Biology·Roser PuigJoan-Francesc Barquinero

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