Aug 1, 1974

Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by mitomycin C

Proceedings of the National Academy of Sciences of the United States of America
S A Latt

Abstract

Sister chromatid exchanges in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with the bisbenzimidazole dye 33258 Hoechst. These exchanges are much more frequent than chromosome or chromatid breaks and appear to be partly but not entirely due to 5-bromodeoxyuridine incorporation. Sister chromatid exchanges are extremely sensitive indicators of chromosome damage produced by DNA cross-linking agents such as mitomycin C. Significant increases in the sister chromatid exchange frequency occur with 3 ng/ml of mitomycin C; higher concentrations of mitomycin C induce further sister chromatid exchanges. Comparatively few gross chromosomal aberrations are seen in cells exhibiting as many as one hundred or more sister chromatid exchanges. Most of the damage caused by mitomycin C to chromosomal DNA is apparently repaired without detectable changes in chromosome morphology. Analysis of sister chromatid exchanges may permit more sensitive detection of damage to DNA caused by other agents than has previously been possible by classical cytological techniques.

  • References17
  • Citations286

References

  • References17
  • Citations286

Citations

Mentioned in this Paper

Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Cytological Techniques
Sister Chromatid Exchange
Science of Morphology
Base Excision Repair
Immunofluorescence Microscopy
Chromosomes, Human
Etiology
Autosome Abnormalities
Chromatids

About this Paper

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.