PMID: 9166756Jun 1, 1997Paper

Site-directed deletion of a 10-nucleotide domain of the 3'-untranslated region of the GLUT1 glucose transporter mRNA eliminates cytosolic protein binding in human brain tumors and induction of reporter gene expression

Journal of Neurochemistry
H TsukamotoWilliam M Pardridge

Abstract

The posttranscriptional regulation of GLUT1 glucose transporter gene expression may be mediated by specific interactions between cytosolic trans-acting factors and regulatory cis-elements within the 3'-untranslated regions (UTRs) of the GLUT1 mRNA. Recent studies demonstrate that experimental and human brain tumors express an 80-kDa protein that reacts with a specific sequence around nucleotide 2,200 within the GLUT1 mRNA 3'-UTR. The 80-kDa protein is selectively expressed in hemangioblastoma, a tumor characterized by overexpression of GLUT1. The enhancer role of this GLUT1 3'-UTR cis-element was confirmed in the present studies using the luciferase expression vector pGL2 and site-directed deletion. Transfection of C6 glioma cells with pGL2 (containing nucleotides 2,100-2,300 of the bovine GLUT1 3'-UTR inserted at the Pfl MI site within the luciferase 3'-UTR) results in a fivefold increase in luciferase gene expression. Deletion of nucleotides 2,181-2,190 of the bovine GLUT1 3'-UTR, i.e., the putative binding site of the 80-kDa protein, completely eliminated the enhancement of luciferase activity in the transfected cells. Luciferase mRNA containing the putative cis-element inserted in the 3'-UTR was transcribed, and after UV cr...Continue Reading

Citations

Jul 30, 2009·American Journal of Physiology. Endocrinology and Metabolism·María F RieraSelva B Cigorraga
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·N ShiW M Pardridge
Sep 24, 1999·Biochemical and Biophysical Research Communications·C Qi, P H Pekala
Jan 8, 1999·Brain Research. Molecular Brain Research·R J Boado, W M Pardridge
Feb 29, 2000·Placenta·N P Illsley

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