PMID: 2117276Aug 1, 1990Paper

Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis

Proceedings of the National Academy of Sciences of the United States of America
M J Weickert, G H Chambliss

Abstract

Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many mutations reduced the repression ratio 3- to 11-fold, some also caused a 2-fold or greater increase in amylase production. Others caused hyperproduction without affecting catabolite repression. Homologous sequences in other catabolite-repressed B. subtilis promoters suggest a common regulatory site may be involved in catabolite repression.

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Citations

Jul 1, 1993·World Journal of Microbiology & Biotechnology·A Puyet
Sep 1, 1993·FEMS Microbiology Reviews·B Poolman
Dec 31, 1990·Biochemical and Biophysical Research Communications·J E GardellaP D Gorevic
Jun 8, 2002·Journal of Molecular Biology·Lwin Mar Aung-HilbrichWolfgang Hillen
Feb 19, 2003·Journal of Molecular Biology·Gregory S AllenRichard G Brennan
May 3, 2000·International Journal of Food Microbiology·C LeboeufA Hartke
Aug 26, 2000·Biochemical Engineering Journal·T MatsumotoH Fukuda
May 3, 2002·Molecular Microbiology·Hyun-Jin KimAbraham L Sonenshein
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·B R Belitsky, A L Sonenshein
Jun 6, 2003·Applied and Environmental Microbiology·Maria Grazia FortinaPier Luigi Manachini
Aug 7, 2003·Applied and Environmental Microbiology·Jacqueline AbranchesRobert A Burne
Oct 6, 2005·Applied and Environmental Microbiology·Tamara Aleksandrzak-PiekarczykJacek Bardowski
Aug 20, 2008·Infection and Immunity·Kati SeidlBrigitte Berger-Bächi
Oct 21, 2009·Infection and Immunity·Colin C Kietzman, Michael G Caparon

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