Site-directed mutagenesis of the VP2 gene of Chicken anemia virus affects virus replication, cytopathology and host-cell MHC class I expression

The Journal of General Virology
Michelle A PetersGlenn F Browning

Abstract

Chicken anemia virus (CAV) is an immunosuppressive pathogen of chickens. To further examine the role of viral protein 2 (VP2), which possesses dual-specificity protein phosphatase (DSP) activity, in viral cytopathogenicity and its influence on viral growth and virulence, an infectious genomic clone of CAV was subjected to site-directed mutagenesis. Substitution mutations C87R, R101G, K102D and H103Y were introduced into the DSP catalytic motif and R129G, Q131P, R/K/K150/151/152G/A/A, D/E161/162G/G, L163P, D169G and E186G into a region predicted to have a high degree of secondary structure. All mutant constructs were infectious, but their growth curves differed. The growth curve for mutant virus R/K/K150/151/152G/A/A was similar to that for wild-type virus, a second cluster of mutant viruses had an extended latent period and a third cluster of mutant viruses had extended latent and eclipse periods. All mutants had a reduced cytopathogenic effect in infected cells and VP3 was restricted to the cytoplasm. Mutation of the second basic residue (K102D) in the atypical DSP signature motif resulted in a marked reduction in virus replication efficiency, whereas mutation of the first basic residue (R101G) attenuated cytopathogenicity, bu...Continue Reading

References

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Aug 2, 2002·The Journal of Biological Chemistry·Michelle A PetersGlenn F Browning
Jan 27, 2004·Veterinary Microbiology·Mathieu H M Noteborn
Feb 22, 2005·The Journal of General Virology·Michelle A PetersGlenn F Browning
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Citations

Jun 29, 2011·Virology Journal·Kun ChenShijun J Zheng
Apr 20, 2011·Avian Diseases·Karel A SchatMichael S Piepenbrink
May 8, 2007·Journal of Virological Methods·Xiaoyan WangXiaomei Wang
Jan 30, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Eliana Ottati Nogueira-DantasLiana Brentano
Feb 16, 2017·Scientific Reports·Shuai YaoXiaomei Wang
Jan 19, 2008·Avian Pathology : Journal of the W.V.P.A·Amir KaffashiGlenn F Browning
Jul 12, 2007·The Journal of General Virology·Michelle A PetersGlenn F Browning

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