PMID: 8599603Feb 29, 1996Paper

Site of action of proteinases in the activation of steroidogenesis in rat adrenal gland

Biochimica Et Biophysica Acta
P G MeleE J Podestá

Abstract

We have investigated the effect of the proteinase inhibitors 1,10-phenantroline (OP) and phenylmethylsulfonyl fluoride (PMSF) on steroidogenesis in rat adrenal cortex. Both PMSF and OP inhibited adrenocorticotropin (ACTH)- and 8-Br cAMP-induced stimulation of corticosterone synthesis. On the contrary, arachidonic acid-induced stimulation of corticosterone synthesis was only slightly inhibited by PMSF and unchanged by OP. Intra- and extracellular cAMP levels were determined by radioimmunoassay. While PMSF did not affect neither the intra- nor the extracellular cAMP levels, OP decreased the intra- and extracellular levels of unstimulated as well as ACTH-stimulated cells. The site of action of the proteinase inhibitors was also studied by recombination of mitochondria with the different subcellular fractions in vitro. Addition of PMSF abolished the stimulation achieved by in vitro activation of cytosol by cAMP and PKA. On the other hand, OP completely inhibited the activation of mitochondria. Our results provide evidence for the involvement of proteinases in ACTH-induced stimulation of steroidogenesis in adrenal cortex both prior to the release of arachidonic acid and at the level of cholesterol transport from the outer to the inn...Continue Reading

References

Jan 1, 1978·Molecular and Cellular Endocrinology·R Neher, A Milani
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P A RaeB P Schimmer
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·B A CookeL Phipp
Dec 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·L A DadaE J Podesta
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R YamamotoJ F Strauss
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A G MukhinK E Krueger
Jan 1, 1984·Endocrine Research·G V VahounyT J Scallen
Feb 15, 1980·The Biochemical Journal·E J PodestaR Neher
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R C Pedersen, A C Brownie

❮ Previous
Next ❯

Citations

Jan 9, 2007·Molecular and Cellular Endocrinology·P MalobertiE J Podestá
Apr 12, 2016·Frontiers in Endocrinology·Carmen Ruggiero, Enzo Lalli
Feb 16, 2006·American Journal of Physiology. Endocrinology and Metabolism·John R PepperellMaricruz Merino
Oct 18, 2020·Cellular and Molecular Life Sciences : CMLS·Ana F CastilloErnesto J Podesta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.