Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles

Organic & Biomolecular Chemistry
Lu Shin WongJason Micklefield

Abstract

The immobilisation of proteins on to nanoparticles has a number of applications ranging from biocatalysis through to cellular delivery of biopharmaceuticals. Here we describe a phosphopantetheinyl transferase (Sfp)-catalysed method for immobilising proteins bearing a small 12-mer "ybbR" tag on to nanoparticles functionalised with coenzyme A. The Sfp-catalysed immobilisation of proteins on to nanoparticles is a highly efficient, single step reaction that proceeds under mild conditions and results in a homogeneous population of proteins that are covalently and site-specifically attached to the surface of the nanoparticles. Several enzymes of interest for biocatalysis, including an arylmalonate decarboxylase (AMDase) and a glutamate racemase (GluR), were immobilised on to nanoparticles using this approach. These enzymes retained their activity and showed high operational stability upon immobilisation.

References

Sep 1, 1985·Journal of Medicinal Chemistry·J C MadelmontG Meyniel
May 20, 1999·Nature Structural Biology·K Y HwangY Cho
Sep 22, 2000·Trends in Biotechnology·S M Moghimi, A C Hunter
Oct 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jun YinChristopher T Walsh
Jan 18, 2007·Bioconjugate Chemistry·Aimee R HerdtT Andrew Taton
Jan 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Samuel K LaiJustin Hanes
Sep 7, 2007·Biomacromolecules·Cleofe PalocciMaria Vittoria Russo
Jun 26, 2008·Chemistry : a European Journal·Krzysztof OkrasaJason Micklefield
Jul 10, 2008·Journal of the American Chemical Society·Kenneth E ScarberryZ John Zhang
Jul 26, 2008·Nature Nanotechnology·Demir AkinRashid Bashir
Jul 26, 2008·Nature Nanotechnology·Dan PeerRobert Langer
Aug 30, 2008·Journal of the American Chemical Society·Lu Shin WongJason Micklefield

❮ Previous
Next ❯

Citations

Nov 21, 2015·Bioconjugate Chemistry·Jan GrunewaldBernhard H Geierstanger
Jul 26, 2012·Biotechnology Journal·Takuya MatsumotoAkihiko Kondo
Nov 28, 2012·Chembiochem : a European Journal of Chemical Biology·Anna-Winona StruckJason Micklefield
Aug 28, 2016·Applied Microbiology and Biotechnology·Kenji Miyamoto, Robert Kourist
Dec 9, 2020·Chembiochem : a European Journal of Chemical Biology·Nan Chen, Chu Wang
Jul 25, 2020·Biochemistry·Rohit H SubramanianF Akif Tezcan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.