Site-selective photoinduced cleavage and profiling of DNA by chiral semiconductor nanoparticles

Nature Chemistry
Maozhong SunHua Kuang

Abstract

Gene editing is an important genetic engineering technique that enables gene manipulation at the molecular level. It mainly relies on engineered nucleases of biological origin, whose precise functions cannot be replicated in any currently known abiotic artificial material. Here, we show that chiral cysteine-modified CdTe nanoparticles can specifically recognize and, following photonic excitation, cut at the restriction site GAT'ATC (' indicates the cut site) in double-stranded DNA exceeding 90 base pairs, mimicking a restriction endonuclease. Although photoinduced reactive oxygen species are found to be responsible for the cleavage activity, the sequence selectivity arises from the affinity between cysteine and the conformation of the specific DNA sequence, as confirmed by quantum-chemical calculations. In addition, we demonstrate non-enzymatic sequence-specific DNA incision in living cells and in vivo using these CdTe nanoparticles, which may help in the design of abiotic materials for gene editing and other biological applications.

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Citations

Apr 6, 2019·Angewandte Chemie·Changlong HaoHua Kuang
Apr 16, 2019·Angewandte Chemie·Jiawei LvZhiyong Tang
Oct 3, 2019·Chemical Communications : Chem Comm·Fang XuJuewen Liu
Dec 14, 2019·Advanced Materials·Sang Won ImKi Tae Nam
Jan 30, 2020·Nanoscale·Jinyi Zhang, Juewen Liu
May 19, 2020·The Analyst·Yufeng LiuHui Wei
Jun 6, 2020·Advanced Materials·Jinchen Fan, Nicholas A Kotov
Jul 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ruiqin Fang, Juewen Liu
Jul 15, 2020·Materials·Motaharesadat Hosseini, Masoud Mozafari
Jan 10, 2020·The Journal of Chemical Physics·Kalil BernardinoMauro C C Ribeiro
Feb 6, 2020·Topics in Current Chemistry·Yuan ZhaoChuanlai Xu
Nov 7, 2019·Advanced Materials·Jihyeon YeomAna Jaklenec
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Feb 10, 2021·Advances in Colloid and Interface Science·Yun WenJian-Hua Wang
Mar 2, 2021·Advanced Materials·Si LiHua Kuang
Jun 5, 2021·Journal of Biomedical Nanotechnology·Heping WangXue Xue
Jun 8, 2021·Chemical Science·Hongyu ZhangHua Kuang
Jul 4, 2021·Advanced Materials·Xin FanRainer Haag
Aug 14, 2019·Journal of the American Chemical Society·Xiaoqing GaoZhiyong Tang
Apr 23, 2019·Inorganic Chemistry·Xiao ShaoShuang Jiang
Oct 11, 2019·The Journal of Physical Chemistry. B·Kalil BernardinoMauro C C Ribeiro

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Methods Mentioned

BETA
transmission
X-ray
electrophoresis
isothermal titration calorimetry
DNA
confocal microscopy
Fluorescence
DNA shearing
transfection

Software Mentioned

Imaging Lab

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