Site-specific analysis of the SARS-CoV-2 glycan shield

BioRxiv : the Preprint Server for Biology
Yasunori WatanabeMax Crispin

Abstract

The emergence of the betacoronavirus, SARS-CoV-2 that causes COVID-19, represents a significant threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, that mediates cell entry and membrane fusion. SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in immune evasion and occluding immunogenic protein epitopes. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design.

Citations

Jul 3, 2020·Journal of Clinical Medicine·Francesco CappelloAlberto Jl Macario
Jun 20, 2020·EMBO Molecular Medicine·Anastasia PolycarpouSteven Sacks
Jun 9, 2020·Biochemical Society Transactions·Spencer J Williams, Ethan D Goddard-Borger
Jan 28, 2021·International Journal of Molecular Sciences·Laura Kate GadanecVasso Apostolopoulos
Feb 16, 2021·Frontiers in Immunology·Anastasia PolycarpouSteven Sacks
Feb 18, 2021·Reviews in Medical Virology·Arash ArashkiaUNKNOWN SARS CoV-2 Rapid Response Team of Pasteur Institute of Iran (PII)
Apr 4, 2021·Medicina·Michael DoulberisJannis Kountouras
Oct 24, 2020·Biochemistry·M Fernando BravoAdam B Braunschweig
Jun 13, 2021·Journal of Biomedical Science·Pei-Shan Sung, Shie-Liang Hsieh
Jun 16, 2021·Genome Medicine·Christof C SmithAlex Rubinsteyn
Mar 23, 2021·Communications Chemistry·Mia I Zol-Hanlon, Benjamin Schumann
Jul 14, 2020·Journal of Proteome Research·Anthony D WhettonNophar Geifman

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Datasets Mentioned

BETA
MN908947

Methods Mentioned

BETA
glycosylation
glycosylate
protein folding
size-exclusion chromatography
electron microscopy

Software Mentioned

TIA
[UNK]

Related Concepts

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