PMID: 7544284Aug 1, 1995Paper

Site-specific and complete enzymic deglycosylation of the native human chorionic gonadotropin alpha-subunit

European Journal of Biochemistry
C W Van ZuylenJ F Vliegenthart

Abstract

Numerous studies have shown that glycosylation of the alpha-subunit of human chorionic gonadotropin (alpha hCG) is essential for the biological activity of this hormone. To obtain detailed insight into the function of N-glycosylation, the availability of site-specifically and fully deglycosylated alpha-subunits obtained under non-denaturing conditions is a prerequisite. NMR spectroscopy in combination with FAB-mapping demonstrates that only Asn52 of the alpha-subunit is accessible to digestion by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F under native conditions. Treatment of native alpha hCG with endo-beta-N-acetylglucosaminidase B results in full deglycosylation yielding alpha hCG with one GlcNAc residue at both Asn52 and Asn78.

References

Jul 1, 1979·Journal of Steroid Biochemistry·G P Talwar
Nov 1, 1992·The Biochemical Journal·A Stockell Hartree, A G Renwick
Nov 15, 1990·The Biochemical Journal·C W Taylor
Jun 1, 1990·The Journal of Endocrinology·J HiyamaA G Renwick
Aug 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J RyanH T Keutmann
Mar 1, 1986·Proceedings of the Society for Experimental Biology and Medicine·J R SwedlowH Papkoff
Nov 15, 1988·Biochemical and Biophysical Research Communications·W E Merz
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R Hardy, R R Townsend
Nov 22, 1967·Journal of the American Chemical Society·C C McDonald, W D Phillips
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Nov 1, 1984·Biomedical Mass Spectrometry·P Roepstorff, J Fohlman
Jun 9, 1994·Nature·A J LapthornN W Isaacs
Dec 1, 1993·Current Biology : CB·C Hammond, A Helenius

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Citations

Dec 20, 1996·Molecular and Cellular Endocrinology·G R BousfieldW T Moore
Aug 16, 2011·Reproductive Biology and Endocrinology : RB&E·Christoph ZenzmaierPeter Berger
Mar 21, 2003·Biochemical and Biophysical Research Communications·Yongna Xing, William R Moyle
Feb 14, 2017·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Johannes F G Vliegenthart
Jul 5, 2007·Molecular Endocrinology·Jean-Michel KrauseWolfgang E Merz
Mar 30, 1999·European Journal of Biochemistry·P J ErbelJ F Vliegenthart
Apr 15, 2000·Methods : a Companion to Methods in Enzymology·G R BousfieldV Y Butnev

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