PMID: 6165013Mar 1, 1981Paper

Site-specific cleavage by T1 RNase of U-1 RNA in u-1 ribonucleoprotein particles

Proceedings of the National Academy of Sciences of the United States of America
P EpsteinH Busch

Abstract

The structures and functions of small nuclear ribonucleoprotein particles have become of interest because of their suggested role in processing heterogeneous nuclear RNA [Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin S. L. & Steitz, J. A. (1980) Nature (London) 283, 220-224]. To determine the conformation of U-1 RNA in U-1 ribonucleoprotein particles and whether proteins of these particles protect segments of U-1 RNA, intact particles and isolated U-1 RNA were digested with T1 RNase. The digested particles were immunoprecipitated with anti-Sm antibodies. A 5'-end fragment containing nucleotides 1-107 and 3'-end fragments containing nucleotides 108-165 and 108-153 were recovered in nearly quantitative yield from digestion of the particles, suggesting that position 107 is the principal cleavage site in them. At the same T1 RNase concentrations, deproteinized U-1 RNA was cleaved into many fragments. At low T1 RNase concentrations, major cleavage site of deproteinized U-1 RNA was at nucleotide 69. Comparison of the cleavage sites of free U-1 RNA and of U-1 RNA in U-1 ribonucleoprotein particles suggested similar secondary structures. The resistance of the 5' end of U-1 RNA to T1 RNase was unexpected inasmuch as this region has b...Continue Reading

References

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Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L J KornM N Wegman
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Jan 10, 1980·Nature·M R LernerJ A Steitz

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Citations

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieH F Noller
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E D WiebenT Pederson
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S L Deutscher, J D Keene
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R Patton, T Pederson
Jul 1, 1983·The Journal of Cell Biology·D J ForbesM W Kirschner
Jan 11, 1984·Nucleic Acids Research·C Queen, L J Korn
Nov 11, 1981·Nucleic Acids Research·R ReddyH Busch
Apr 15, 1988·European Journal of Biochemistry·N Brajanović-UrosevićV Holoubek
Nov 30, 1982·Biochemical and Biophysical Research Communications·P EpsteinH Busch
May 15, 1985·Analytical Biochemistry·W H SchrierT B Okarma
Jan 29, 1982·Biochemical and Biophysical Research Communications·J Sri-WidadaC Brunel
Sep 1, 1984·Bioscience Reports·R W Davies
Jul 1, 1990·Molecular and Cellular Biology·H E Neuman de Vegvar, J E Dahlberg

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