Jul 9, 1984

Site-specific cleavage of double-strand DNA by hydroperoxide of linoleic acid

FEBS Letters
S Inouye


The breakage of double-strand (ds) DNA by 13-L-hydroperoxy-cis-9,trans-11-octadecadienoic acid (LAHPO) was investigated by agarose gel electrophoresis of supercoiled pBR322 DNA and the site of cleavage on the DNA molecule was determined by the method of DNA sequence analysis using 3'-end and 5'-end-labeled DNA fragments as substrates. LAHPO caused cleavage at the position of guanine nucleotide in dsDNA. LAHPO caused dsDNA breaks at specific sites, but linoleic acid (LA) and 13-L-hydroxy-cis-9,trans-11-octadecadienoic acid (LAHO) have no such effects on dsDNA. The active oxygen atom of the hydroperoxy group of LAHPO was perhaps responsible for the site-specific cleavage of dsDNA.

Mentioned in this Paper

Hydrogen Peroxide
Electrophoresis, Agar Gel
octadecadienoic acid
Cytokinesis of the Fertilized Ovum
Lipid Peroxides
Linoleic Acids
13-hydroxy-9,11-octadecadienoic acid, (Z, E)-isomer

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