PMID: 9528657Apr 7, 1998Paper

Site-specific cleavage of tRNA by imidazole and/or primary amine groups bound at the 5'-end of oligodeoxyribonucleotides

Biochimica Et Biophysica Acta
K UshijimaH Takaku

Abstract

Sequence specific RNA cleaving molecules were synthesized by attaching novel polyamine derivatives bearing imidazole and/or primary amine groups to the 5'-end of DNA oligonucleotides as the sequence-recognizing moieties. The actions of the molecules on a half-tRNA(Asp) were investigated. The oligonucleotides directed the nuclease activity (the imidazole and the primary amine are the catalytic groups) of the enzyme to the nucleotides directly adjacent to the complementary target sequence on the substrate RNA. The cleavage reaction shows a bell-shaped pH dependence with a maximum at pH 7.0, indicating the participation of protonated and non-protonated imidazoles residues in the process. The specificity of these hybrid enzymes can be easily altered, and they should prove to be useful tools for probing RNA structures in solution and as potential reactive groups in antisense oligonucleotide derivatives. We also describe the site-specific cleavage of tRNA(Asp) by the cleaving reagents bearing imidazole and/or primary amine groups at the 5'-end of oligodeoxyribonucleotides.

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Citations

Jan 23, 1999·Current Opinion in Chemical Biology·M Komiyama, J Sumaoka
Apr 15, 2011·Nucleosides, Nucleotides & Nucleic Acids·Anthony D Saleh, Paul S Miller
Mar 29, 2007·Nucleic Acids Research·N L MironovaM A Zenkova
Apr 16, 2013·Biochemical and Biophysical Research Communications·Liang XuKeliang Liu
Apr 17, 2001·Nucleosides, Nucleotides & Nucleic Acids·K ShinozukaH Sawai
Jul 8, 2008·Chembiochem : a European Journal of Chemical Biology·Jesus RazkinLars Baltzer
Dec 17, 2005·Organic & Biomolecular Chemistry·Teija Niittymäki, Harri Lönnberg
Apr 18, 2006·Journal of Biomolecular Structure & Dynamics·Nadezhda L MironovaValentin V Vlassov
Aug 22, 2002·Journal of the American Chemical Society·Leonard LermerDavid M Perrin

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