PMID: 9169572Jun 1, 1997Paper

Site-specific conjugation and labelling of prostate antibody 7E11C5.3 (CYT-351) with technetium-99m

European Journal of Nuclear Medicine
M A StalteriK E Britton

Abstract

Attachment of chelating agents to the sugar residues of antibodies for subsequent radiolabelling is an attractive approach since it may have less effect on the immunoreactivity than attachment through lysine residues, which are distributed throughout the antibody and may be present near the antigen binding site. We have attached a new hydrazide-linked chelator CYT-395 (Cytogen Corp., Princeton, N.J.) to the sugar residues of the anti-prostate monoclonal antibody 7E11C5.3 and optimised the conditions for labelling the conjugate with technetium-99m in order to compare the conjugate to 7E11C5.3 antibody labelled directly with technetium using a mercaptoethanol reduction technique. Labelling yields of 70%-90% were obtained at specific activities up to 2000 MBq/mg antibody. The stability of the technetium-labelled conjugate in plasma or to a challenge with 0.1 or 1.0 mM cysteine was similar to that of direct-labelled antibody. In nine patients with prostate cancer, the plasma clearance of the labelled conjugate followed a two-compartment model, with an average beta-phase half-life of 31.4+/-3.9 h. The average urinary clearance at 24 h was 15.3+/-5.0% of the injected dose. In this group of patients there was no significant difference...Continue Reading

References

Nov 5, 1991·Journal of Immunological Methods·R AbrahamK E Hellström
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J D RodwellT J McKearn
Nov 1, 1994·Nuclear Medicine and Biology·D J HnatowichP Winnard

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Citations

Jan 13, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Pierre AdumeauBrian M Zeglis

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