PMID: 9177202Jun 10, 1997Paper

Site-specific de-N-glycosylation of diglycosylated ovalbumin in hen oviduct by endogenous peptide: N-glycanase as a quality control system for newly synthesized proteins

Proceedings of the National Academy of Sciences of the United States of America
T SuzukiS Inoue

Abstract

Hen ovalbumin (OVA) is known to exist as a singly N-glycosylated form with a glycan chain on Asn-292 in egg white. Previous studies showed that di-N-glycosylated form of OVA [Di-OVA; CHO-Asn-292/CHO-Asn-311 (CHO, N-glycan chain)], which has two N-glycan chains on Asn-292 and Asn-311, was expressed only transiently in hen oviduct. Di-OVA was not found in egg white, suggesting that this form cannot be secreted normally and may possibly be converted to mono-N-glycosylated OVA (CHO-Asn-292/Asp-311) by the action of peptide:N-glycanase (PNGase) during synthesis and secretion. In this study, we have identified the putative PNGase activity in the homogenate of hen oviduct, purified 1,000-fold, and designated as PNGase HO. We examined the reactivity of Di-OVA to PNGase HO and found that this enzyme site-specifically cleaved off the glycan chain at Asn-311 to convert Di-OVA into the mono-N-glycosylated form (CHO-Asn-292/Asp-311). In contrast, this enzyme was found not to act on the mono-N-glycosylated OVA (CHO-Asn-292/Asn-311) found in egg white when it was tested as a substrate. The present findings support our view that de-N-glycosylation catalyzed by PNGase may be involved in quality control of newly synthesized proteins by convertin...Continue Reading

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Citations

Oct 6, 2001·Biochemical and Biophysical Research Communications·T SuzukiW J Lennarz
Mar 30, 2007·Analytical and Bioanalytical Chemistry·Karin OrtnerNorbert Müller
Aug 10, 2006·Glycoconjugate Journal·Tadashi Suzuki, Yoko Funakoshi
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Tadashi Suzuki
Feb 21, 2003·Biochemical and Biophysical Research Communications·Tadashi Suzuki, William J Lennarz
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·J A Huntington, P E Stein
Jan 5, 1999·Bioorganic & Medicinal Chemistry Letters·I L DerasY C Lee
Mar 28, 2013·Chemical Reviews·William R AlleyMilos V Novotny
Feb 13, 2010·The Journal of Biological Chemistry·Hiroto HirayamaTadashi Suzuki
Aug 2, 2005·Molecular Biology of the Cell·Samiksha KatiyarWilliam J Lennarz
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·H ParkW J Lennarz
Sep 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Samiksha KatiyarWilliam J Lennarz
Apr 26, 2005·Bioscience, Biotechnology, and Biochemistry·Kazunari Ito, Naotoshi Matsudomi
Jun 1, 2000·The Journal of Cell Biology·T SuzukiW J Lennarz
Oct 28, 2008·Biochimica Et Biophysica Acta·Yoko Funakoshi, Tadashi Suzuki
Oct 24, 2007·Seminars in Cell & Developmental Biology·Tadashi Suzuki
May 19, 2007·Biochemical and Biophysical Research Communications·Tadashi SuzukiAntonio Colavita
Sep 8, 2004·Biochemical and Biophysical Research Communications·Shyamasri BiswasHermann Schindelin
Dec 6, 2014·Seminars in Cell & Developmental Biology·Hiroto HirayamaTadashi Suzuki
Mar 17, 2015·Cellular and Molecular Life Sciences : CMLS·Yoichiro HaradaTadashi Suzuki
May 30, 1998·The Journal of Biological Chemistry·T SuzukiW J Lennarz
Nov 1, 2006·Molecular & Cellular Proteomics : MCP·Bingyun SunLeroy Hood
Apr 15, 2017·Biochemical Society Transactions·Dominic S AlonziNicole Zitzmann
Mar 22, 2001·The Journal of Biological Chemistry·T SuzukiW J Lennarz
Apr 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tadashi SuzukiWilliam J Lennarz
Aug 5, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Simone C de CassanSimon J Draper

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