Site-Specific Difunctionalization of Structured RNAs Yields Probes for microRNA Maturation

Bioconjugate Chemistry
Ugo Pradere, Jonathan Hall

Abstract

Modified oligonucleotides bearing multiple functional labels are valuable tools in RNA biology. Efficient synthetic access to singly modified short DNAs and RNAs has been developed in the past years and paved the way to a first generation of oligonucleotide tools. Here, we describe an efficient procedure for the site-specific hetero bis-labeling of long RNAs. We exemplified the method with the preparation of Cy3/Cy5 pre-microRNAs labeled at selected internal sites as probes for microRNA maturation.

References

Jun 24, 2005·Nature·Thimmaiah P ChendrimadaRamin Shiekhattar
Jan 21, 2006·The EMBO Journal·Yoontae LeeV Narry Kim
Jul 22, 2006·Angewandte Chemie·Brian P Davies, Christoph Arenz
Dec 7, 2007·The Journal of Organic Chemistry·Anup M JawalekarFloris L van Delft
Dec 18, 2007·Chemistry & Biodiversity·Margarita Alvira, Ramon Eritja
Apr 3, 2008·Angewandte Chemie·Philipp M E GramlichThomas Carell
Aug 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Asif IqbalDavid M J Lilley
Sep 25, 2008·Angewandte Chemie·Philipp M E GramlichThomas Carell
Oct 31, 2008·Journal of Fluorescence·Billie J Harvey, Marcia Levitus
Jan 7, 2009·The Journal of Organic Chemistry·Gwladys PourceauFrançois Morvan
Apr 30, 2009·Annual Review of Biomedical Engineering·Gang BaoAndrew Tsourkas
Mar 24, 2010·Chemical Society Reviews·Afaf H El-Sagheer, Tom Brown
Jul 28, 2010·Nucleic Acids Research·Xiaoxiao Zhang, Yan Zeng
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Afaf H El-Sagheer, Tom Brown
Dec 7, 2010·Chembiochem : a European Journal of Chemical Biology·Eduardo Paredes, Subha R Das
May 5, 2011·Bioconjugate Chemistry·Anu KiviniemiHarri Lönnberg
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Afaf H El-SagheerTom Brown
Apr 7, 2012·The Journal of Organic Chemistry·Suresh S Pujari, Frank Seela
Aug 16, 2012·Chemical Communications : Chem Comm·Montserrat ShelbourneTom Brown
Sep 1, 2012·Chembiochem : a European Journal of Chemical Biology·Søren Preus, L Marcus Wilhelmsson
Oct 6, 2012·Chemical Communications : Chem Comm·Salifu Seidu-LarryOlwen Domingo
Sep 27, 2013·Nucleic Acids Research·Luca F R GebertJonathan Hall
Oct 12, 2013·Angewandte Chemie·Ugo PradèreJonathan Hall
Dec 24, 2013·Bioconjugate Chemistry·Tobias SantnerRonald Micura
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim

❮ Previous
Next ❯

Citations

Oct 21, 2016·Bioconjugate Chemistry·Martina JezowskaMalgorzata Honcharenko
Mar 16, 2017·Chemistry : a European Journal·Ugo PradèreJonathan Hall
Jun 10, 2016·Organic & Biomolecular Chemistry·Antoine MaruaniVijay Chudasama
Jul 24, 2020·International Journal of Molecular Sciences·Olga A KrashenininaAlya G Venyaminova
Nov 3, 2016·Chembiochem : a European Journal of Chemical Biology·Mirjam MenziJonathan Hall
Jan 19, 2021·ACS Omega·Dmytro HoncharenkoRoger Strömberg
Aug 29, 2019·ACS Omega·Timo HagenJonathan Hall
Jun 12, 2021·Chemistry : a European Journal·Anaïs DepaixJoanna Kowalska
Apr 3, 2019·ACS Central Science·Brennan J WalderLyndon Emsley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved