Site-Specific Dynamics of β-Sheet Peptides with (D) Pro-Gly Turns Probed by Laser-Excited Temperature-Jump Infrared Spectroscopy

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Alexander PoppKarin Hauser

Abstract

Turn residues and side-chain interactions play an important role for the folding of β-sheets. We investigated the conformational dynamics of a three-stranded β-sheet peptide ((D) P(D) P) and a two-stranded β-hairpin (WVYY-(D) P) by time-resolved temperature-jump (T-jump) infrared spectroscopy. Both peptide sequences contain (D) Pro-Gly residues that favor a tight β-turn. The three-stranded β-sheet (Ac-VFITS(D) PGKTYTEV(D) PGOKILQ-NH2 ) is stabilized by the turn sequences, whereas the β-hairpin (SWTVE(D) PGKYTYK-NH2 ) folding is assisted by both the turn sequence and hydrophobic cross-strand interactions. Relaxation times after the T-jump were monitored as a function of temperature and occur on a sub-microsecond time scale, (D) P(D) P being faster than WVYY-(D) P. The Xxx-(D) Pro tertiary amide provides a detectable IR band, allowing us to probe the dynamics site-specifically. The relative importance of the turn versus the intrastrand stability in β-sheet formation is discussed.

References

Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P A AnfinrudR M Hochstrasser
Jan 23, 1999·Current Opinion in Chemical Biology·S H Gellman
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·D T ClarkeG R Jones
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·A G CochranM A Starovasnik
Sep 20, 2001·Journal of the American Chemical Society·C Y HuangF Gai
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·H E StangerS H Gellman
Aug 9, 2002·Journal of the American Chemical Society·Chad D Tatko, Marcey L Waters
Jun 19, 2003·Journal of the American Chemical Society·Jovencio HilarioTimothy A Keiderling
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rita P-Y ChenSunney I Chan
Dec 1, 2005·Journal of the American Chemical Society·Nicole N-W KuoSunney I Chan
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Stefania AbbruzzettiJohn E T Corrie
Jul 14, 2006·Current Opinion in Structural Biology·Robert M Hughes, Marcey L Waters
Dec 7, 2006·Journal of the American Chemical Society·Yao XuFeng Gai
Jan 13, 2011·The Journal of Physical Chemistry. B·Mateusz L DontenJoost VandeVondele
Feb 23, 2013·BMJ Case Reports·Peter Ng
Mar 13, 2014·The Journal of Physical Chemistry. B·Beatrice N MarkiewiczFeng Gai
Nov 14, 2014·The Journal of Physical Chemistry. B·Alexander PoppKarin Hauser
Dec 30, 2014·The Journal of Physical Chemistry. B·Mateusz L DontenPeter Hamm

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