Site-specific fluorescent labeling of nascent proteins on the translating ribosome

Journal of the American Chemical Society
Ishu SaraogiShu-Ou Shan

Abstract

As newly synthesized proteins emerge from the ribosome, they interact with a variety of cotranslational cellular machineries that facilitate their proper folding, maturation, and localization. These interactions are essential for proper function of the cell, and the ability to study these events is crucial to understanding cellular protein biogenesis. To this end, we have developed a highly efficient method to generate ribosome-nascent chain complexes (RNCs) site-specifically labeled with a fluorescent dye on the nascent polypeptide. The fluorescent RNC provides real-time, quantitative information on its cotranslational interaction with the signal recognition particle and will be a valuable tool in elucidating the role of the translating ribosome in numerous biochemical pathways.

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Citations

Feb 11, 2012·Analytical and Bioanalytical Chemistry·Hui-wang Ai
Nov 13, 2012·Nature Structural & Molecular Biology·Wolf HoltkampWolfgang Wintermeyer
Jan 10, 2012·The Journal of Biological Chemistry·Dawei Zhang, Shu-Ou Shan
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Feb 19, 2013·Annual Review of Biochemistry·David AkopianShu-Ou Shan
Jun 5, 2014·Annual Review of Biophysics·Xin Zhang, Shu-Ou Shan
Jun 11, 2014·The Journal of Cell Biology·Ishu SaraogiShu-Ou Shan
Feb 13, 2013·The Journal of Cell Biology·David AkopianShu-Ou Shan
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Aileen AriosaShu-Ou Shan
Feb 12, 2014·Biochimica Et Biophysica Acta·Ishu Saraogi, Shu-ou Shan
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Oct 2, 2019·Nature Structural & Molecular Biology·Shuai WangShu-Ou Shan
May 20, 2020·Organic & Biomolecular Chemistry·Patrick R GleasonJeremy H Mills

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