PMID: 3753454Jun 1, 1986Paper

Site-specific modification of albumin by free radicals. Reaction with copper(II) and ascorbate

The Biochemical Journal
G Marx, M Chevion

Abstract

Exposure of albumin to Cu(II) (10-100 microM) and ascorbate (0.1-2 mM) results in extensive molecular modifications, indicated by decreased fluorescence and chain breaks. The rate of utilization of molecular oxygen and ascorbate as a function of Cu(II) concentration is non-linear at copper/albumin ratios of greater than 1. It appears that Cu(II) bound to the tightest albumin-binding site is less available to the ascorbate than the more loosely bound cation. SDS/polyacrylamide-gel electrophoresis reveals new protein bands corresponding to 50, 47, 22, 18 and 3 kDa. For such a cleavage pattern, relatively few (approximately 3) and rather specific chain breaks occurred. Repeated addition of portions of ascorbate to the albumin/Cu(II) mixture results in increased intensity of the new bands. The absence of Cu(II) or the presence of metal chelating agents is inhibitory. There was no evidence of intermolecular cross-linking or of the formation of insoluble, albumin-derived, material. A mechanism is proposed wherein the loosely bound Cu(II) participates in a Fenton-type reaction. This generates OH. radicals, which rapidly inter-react with the protein and modify it in a 'site-specific' manner.

Citations

Aug 20, 1990·FEBS Letters·J V Hunt, S P Wolff
Jan 1, 1991·Free Radical Research Communications·G Marx
Jan 1, 1990·Free Radical Research Communications·B Halliwell
Jan 1, 1989·Free Radical Research Communications·R T DeanM A McElligott
Jan 15, 1995·European Journal of Biochemistry·B ChanR Harris
Apr 1, 1996·Photochemistry and Photobiology·G MarxB Horowitz
Sep 12, 2009·Drug Metabolism and Pharmacokinetics·David C Gaze
Feb 12, 2019·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·Naci BabatHalit Demir
Jan 1, 1991·Free Radical Research Communications·K E Lovering, R T Dean
Jan 1, 1991·Free Radical Research Communications·P Di SimplicioT F Slater
Feb 1, 1997·Biological Trace Element Research·D A Clopton, P Saltman
Mar 24, 2007·European Biophysics Journal : EBJ·Edit SchnitzerDov Lichtenberg
Dec 29, 2004·Free Radical Research·Dorit Samocha-BonetIlya Pinchuk
Jun 9, 2007·Acta Veterinaria Hungarica·Suzana Milinković-TurBojana Gradinski-Vrbanac
Jun 27, 2014·Indian Journal of Clinical Biochemistry : IJCB·Prema GurumurthyK M Cherian
Mar 31, 2016·The Biochemical Journal·Michael J Davies
Apr 1, 1997·Redox Report : Communications in Free Radical Research·J M Gebicki
Jan 22, 2017·Analytical and Bioanalytical Chemistry·Fobang LiuChristopher J Kampf
Jan 1, 1991·Free Radical Research Communications·J V Hunt, S P Wolff
Nov 18, 2004·The Journal of Biological Chemistry·Xianlong GaoTarun B Patel
Aug 30, 2008·Journal of Clinical Pathology·P O Collinson, D C Gaze
Oct 30, 2012·Toxicology and Industrial Health·Sahar Y Al-Okbi
Mar 15, 2012·Chembiochem : a European Journal of Chemical Biology·Krista Rule WiggintonTamar Kohn
May 10, 2013·Critical Reviews in Toxicology·Dennis J PaustenbachBrent D Kerger
Apr 30, 2013·Expert Opinion on Drug Metabolism & Toxicology·Zemek FilipKuca Kamil
May 24, 2020·Antioxidants·Ema Valentina BrovčJanez Mravljak
Nov 6, 1989·European Journal of Biochemistry·V M FernandezE C Hatchikian
Jul 13, 2017·Oxidative Medicine and Cellular Longevity·Ilaria MarroccoIlaria Peluso
Apr 14, 2015·Veterinary Clinical Pathology·Amy N SchnellePhilip Solter
Jan 25, 2021·Free Radical Biology & Medicine·David H GonzalezSuzanne E Paulson

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