Site-specific modification of positively-charged surfaces on human serum albumin by malondialdehyde

Biochemical and Biophysical Research Communications
Takeshi IshiiKoji Uchida

Abstract

Malondialdehyde (MDA), a lipid peroxidation product, reacts with lysine residues in proteins. Human serum albumin (HSA) is a major target of MDA-modification of serum proteins. To identify, the modification sites of HSA by MDA in vitro, MDA-treated HSA was digested with a protease and the resulting peptides were subjected to liquid chromatography-tandem mass spectrometry. We identified six peptides, which contained a N-propenal adduct at Lys136, Lys174, Lys240, Lys281, Lys525, and Lys541, and revealed that Lys525 is the most reactive residue for MDA modification. Analysis of electrostatic surface potential of a 3-D model structure of HSA indicates that Lys525 is located at the center of positively charged grooves. The results of this study indicate that the modification of proteins by lipid-derived aldehydes may be influenced by the electrostatic potential of the protein surface.

References

Jul 16, 1992·Nature·X M He, D C Carter
Sep 1, 1990·Chemical Research in Toxicology·K StoneL J Marnett
Mar 16, 1989·Nature·K M ShokatP G Schultz
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M E HaberlandP A Edwards
May 26, 1995·Science·B Honig, A Nicholls
Aug 18, 2000·Free Radical Biology & Medicine·K Uchida
Aug 18, 2000·Free Radical Biology & Medicine·J W Baynes, S R Thorpe
Feb 13, 2001·Free Radical Biology & Medicine·D VayE Albano
Jan 5, 2002·Free Radical Biology & Medicine·Elisa MottaranEmanuele Albano
Oct 23, 2003·The Journal of Biological Chemistry·David A SlatterAllen J Bailey
Feb 12, 2004·Bioinformatics·Kengo Kinoshita, Haruki Nakamura
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Duncan E McRee
Dec 4, 2004·Protein Engineering, Design & Selection : PEDS·Rajasri BhattacharyyaPinak Chakrabarti
Aug 2, 2005·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Daniele Del RioNicoletta Pellegrini
Sep 20, 2005·Journal of Molecular Biology·Jamie GhumanStephen Curry
Jan 18, 2006·Chemical Research in Toxicology·Takeshi IshiiKoji Uchida
Apr 26, 2006·Clinical and Experimental Immunology·J R PalacioP Martínez
Nov 9, 2006·Free Radical Research·Koji Uchida
Feb 1, 2007·Redox Report : Communications in Free Radical Research·Giancarlo AldiniMarina Carini

❮ Previous
Next ❯

Citations

Feb 27, 2013·Journal of Proteomics·Neven ZarkovicKamelija Zarkovic
Sep 29, 2009·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Joan Lopez HellinJose L Nieto
Mar 7, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Adam T Slósarczyk, Lars Baltzer
Mar 26, 2016·Journal of Chemical Theory and Computation·Natalia Diaz, Dimas Suarez
May 29, 2015·Journal of Agricultural and Food Chemistry·Angelique Vandemoortele, Bruno De Meulenaer
Jul 10, 2012·Experimental and Molecular Pathology·Susanna MillantaNicola Traverso
Mar 29, 2019·JCI Insight·Maarten M SteinzJohanna T Lanner
Apr 25, 2020·JAD Reports·Francis H C TsaoKeith C Meyer
Sep 9, 2017·Scientific Reports·Jun NakamuraScott J Bultman
Sep 29, 2017·Rheumatology·Ted R MikulsGeoffrey M Thiele
Mar 7, 2021·Antioxidants·Álvaro Viedma-PoyatosDolores Pérez-Sala
Jun 10, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ivan M KempsonTimothy J Barnes
Sep 12, 2014·Chemical Research in Toxicology·Sarah C ShuckLawrence J Marnett
Sep 29, 2020·Journal of Agricultural and Food Chemistry·Angelique VandemoorteleBruno De Meulenaer
Nov 14, 2021·Archives of Toxicology·Gabriele Sabbioni, Billy W Day

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.