PMID: 3748049Sep 1, 1986Paper

Site-specific mutagenesis in vivo by single methylated or deaminated purine bases

Mutation Research
M Hill-PerkinsP Karran

Abstract

The technique of site-directed mutagenesis has been used to investigate the mutagenicity of O6-methylguanine (O6-MeG) or hypoxanthine introduced as a single lesion at a specific locus in an M13mp9 RF molecule constructed in vitro. Following transformation of O6-MeG-containing RF molecules into E. coli JM101, mutant progeny phage were produced at a frequency not significantly different from that observed with wild-type M13mp9 RF. The mutant yield was greatly enhanced by exhausting cellular O6-MeG DNA-methyltransferase before transformation. In contrast, hypoxanthine exhibited miscoding mutagenesis in the absence of interference with cellular repair mechanisms. This indicates that cellular hypoxanthine-DNA glycosylase acts inefficiently in the removal of hypoxanthine from DNA in vivo. The precise mutational changes induced by hypoxanthine were determined by DNA sequence analysis.

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Citations

Aug 1, 1998·Mutation Research·B J GlassnerL D Samson
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·D ParkhomchukV Ogryzko
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gerard MazonRobert P Fuchs
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bo PangPeter C Dedon
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K S EllisonJ M Essigmann
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Saparbaev, J Laval
Sep 12, 2012·Journal of Biomolecular Structure & Dynamics·Ol'ha O Brovarets', Dmytro M Hovorun
Aug 4, 2005·Nucleic Acids Research·Valeria SimonelliPaola Fortini
Sep 6, 2006·Journal of Bacteriology·Brian Budke, Andrei Kuzminov
May 6, 2003·Journal of Bacteriology·Nicholas E BurgisRichard P Cunningham
May 9, 2003·BMC Biochemistry·Roichi ItohBertrand Daignan-Fornier
Mar 4, 2005·Biological & Pharmaceutical Bulletin·Mika HoriHiroyuki Kamiya
Sep 26, 2015·Biomolecules·Isao Kuraoka
Apr 1, 2015·Progress in Biophysics and Molecular Biology·Bjørn DalhusMagnar Bjørås
Sep 12, 2015·Cell & Bioscience·Chia-Chia LeeWoei-Horng Fang
Jul 28, 2009·Journal of Molecular Biology·Elena I StepchenkovaYouri I Pavlov
Jun 5, 2013·Journal of Biomolecular Structure & Dynamics·Ol'ha O Brovarets'Dmytro M Hovorun
Apr 1, 1989·Journal of the American College of Nutrition·D H Barch

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