PMID: 7520092Sep 1, 1994Paper

Site-specific RNA binding by a hepatitis B virus reverse transcriptase initiates two distinct reactions: RNA packaging and DNA synthesis

Journal of Virology
J R Pollack, D Ganem

Abstract

Hepatitis B viruses encode a polymerase (P) protein with key roles in both reverse transcription and genomic RNA encapsidation. Genetic analysis of cis-acting signals required for viral replication implicates an RNA stem-loop structure in both RNA packaging and the initiation of reverse transcription, a process in which P protein also serves as the primer. We now show that duck hepatitis B virus (DHBV) polymerase binds specifically and with high affinity to this RNA stem-loop structure. Mutational analysis indicates that all mutations in the RNA target that inhibit the P protein-RNA interaction inhibit both in vivo RNA packaging and in vitro DNA priming to comparable extents. However, certain mutations in the loop region of the RNA have minimal impact on P protein-RNA binding but are nonetheless severely defective for packaging and DNA synthesis. Thus, P protein-RNA complex formation is necessary but not sufficient to initiate these activities. In addition, examination of RNA binding by truncated P proteins indicates that the C terminus of the polymerase, although required for RNA encapsidation in vivo, is dispensable for RNA binding and DNA priming.

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Citations

Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
May 3, 2002·The Journal of Biological Chemistry·Xingtai WangJianming Hu
Aug 21, 2002·Journal of Virology·Kristin M Ostrow, Daniel D Loeb
Nov 22, 2002·The Journal of Biological Chemistry·Ermei Yao, John E Tavis
Oct 29, 2002·The Journal of Biological Chemistry·Shiow-Yi ChenYan-Hwa Wu Lee
Dec 15, 2005·Journal of Virology·John F ReganTristram G Parslow
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Sep 1, 1996·Journal of Viral Hepatitis·M Nassal, H Schaller
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M Janda, P Ahlquist
Jul 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Stefan F WielandFrancis V Chisari
Sep 29, 2006·The Journal of Biological Chemistry·Zhian Zhang, John E Tavis
Feb 28, 2008·Journal of Cellular Physiology·David H NguyenJianming Hu
Oct 13, 2006·Biological Procedures Online·Matthew P BadtkeJohn E Tavis
Dec 19, 1998·Journal of Viral Hepatitis·A Kramvis, M C Kew
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·S UrbanD L Tyrrell
Apr 1, 1997·Pathology International·T UchidaH Shinzawa
Mar 12, 2004·The New England Journal of Medicine·Don Ganem, Alfred M Prince
Jun 1, 2010·Viruses·Erik De ClercqJohan Neyts

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