Site-specific selfish genes exploit host functions to copy themselves into a defined target DNA sequence, and include homing endonuclease genes, group II introns and some LINE-like transposable elements. If such genes can be engineered to target new host sequences, then they can be used to manipulate natural populations, even if the number of individuals released is a small fraction of the entire population. For example, a genetic load sufficient to eradicate a population can be imposed in fewer than 20 generations, if the target is an essential host gene, the knockout is recessive and the selfish gene has an appropriate promoter. There will be selection for resistance, but several strategies are available for reducing the likelihood of it evolving. These genes may also be used to genetically engineer natural populations, by means of population-wide gene knockouts, gene replacements and genetic transformations. By targeting sex-linked loci just prior to meiosis one may skew the population sex ratio, and by changing the promoter one may limit the spread of the gene to neighbouring populations. The proposed constructs are evolutionarily stable in the face of the mutations most likely to arise during their spread, and strategies a...Continue Reading
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piggybac- and PhiC31-mediated genetic transformation of the Asian tiger mosquito, Aedes albopictus (Skuse)
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A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae
Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti
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Antipathogen genes and the replacement of disease-vectoring mosquito populations: a model-based evaluation
Evolutionary decay and the prospects for long-term disease intervention using engineered insect vectors
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The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold
A 971-bp insertion in the rns gene is associated with mitochondrial hypovirulence in a strain of Cryphonectria parasitica isolated from nature
Understanding the DNA damage response in order to achieve desired gene editing outcomes in mosquitoes
Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
Impact of mosquito gene drive on malaria elimination in a computational model with explicit spatial and temporal dynamics
Mitonuclear interactions, mtDNA-mediated thermal plasticity, and implications for the Trojan Female Technique for pest control
The use of driving endonuclease genes to suppress mosquito vectors of malaria in temporally variable environments
The promise and peril of CRISPR gene drives: Genetic variation and inbreeding may impede the propagation of gene drives based on the CRISPR genome editing technology
Teilhard de Chardin's oeuvre within an ongoing discussion of a gene drive release for public health reasons
Pathway to Deployment of Gene Drive Mosquitoes as a Potential Biocontrol Tool for Elimination of Malaria in Sub-Saharan Africa: Recommendations of a Scientific Working Group†
Genetically engineered mosquitoes, Zika and other arboviruses, community engagement, costs, and patents: Ethical issues
Pathogen-insect interaction candidate molecules for transmission-blocking control strategies of vector borne diseases
A CRISPR-Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes
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Global Succulent Biome phylogenetic conservatism across the pantropical Caesalpinia Group (Leguminosae)
Dodging silver bullets: good CRISPR gene-drive design is critical for eradicating exotic vertebrates
Agricultural pest control with CRISPR-based gene drive: time for public debate: Should we use gene drive for pest control?
Developing CRISPR-based sex-ratio distorters for the genetic control of fruit fly pests: A how to manual
Fitness consequences of a non-recombining sex-ratio drive chromosome can explain its prevalence in the wild
Antiviral Effectors and Gene Drive Strategies for Mosquito Population Suppression or Replacement to Mitigate Arbovirus Transmission by Aedes aegypti
Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi
Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella
Development and testing of a novel killer-rescue self-limiting gene drive system in Drosophila melanogaster
The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito
Nix alone is sufficient to convert female Aedes aegypti into fertile males and myo-sex is needed for male flight
Translating gene drive science to promote linguistic diversity in community and stakeholder engagement
Genome variation and population structure among 1142 mosquitoes of the African malaria vector species Anopheles gambiae and Anopheles coluzzii
The developmental transcriptome of the mosquito Aedes aegypti, an invasive species and major arbovirus vector
Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research
Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases
Motivations and expectations driving community participation in entomological research projects: Target Malaria as a case study in Bana, Western Burkina Faso
A CRISPR homing gene drive targeting a haplolethal gene removes resistance alleles and successfully spreads through a cage population
Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti
The effect of silencing immunity related genes on longevity in a naturally occurring Anopheles arabiensis mosquito population from southwest Ethiopia
High-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation
Gene drive and resilience through renewal with next generation Cleave and Rescue selfish genetic elements
Climatic Conditions: Conventional and Nanotechnology-Based Methods for the Control of Mosquito Vectors Causing Human Health Issues
Vector genetics, insecticide resistance and gene drives: An agent-based modeling approach to evaluate malaria transmission and elimination
Population-level multiplexing: A promising strategy to manage the evolution of resistance against gene drives targeting a neutral locus
Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs
Tethered homing gene drives: A new design for spatially restricted population replacement and suppression
The need for new vector control approaches targeting outdoor biting Anopheline malaria vector communities
Gene drives in our future: challenges of and opportunities for using a self-sustaining technology in pest and vector management
Potential of gene drives with genome editing to increase genetic gain in livestock breeding programs
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