PMID: 5966173Jul 1, 1966

Sites of glycogen synthesis in rat liver cells as shown by electron microscope radioautography after administration of glucose-H3

The Journal of Cell Biology
A Coimbra, C P Leblond


Glycogen synthesis was investigated by giving tritium (H(3))-labeled glucose with carrier to fasted rats in vivo or incubating liver slices from fasted rats in vitro using a glucose-H(3)-containing medium. After 15 min or 1 hr, pieces of liver were fixed and radioautographed for light and electron microscopy. In vivo and in vitro, radioautographic reactions appeared over "glycogen areas" and over zones transitional between these areas and ergastoplasm. Treatment of sections by alpha amylase removed all but about 5% of the radioactivity, so that about 95% of it consisted of glycogen (synthesized during the 15 min or 1 hr elapsing after administration of glucose-H(3)). Within glycogen areas and transitional zones, most silver grains were over or very close to glycogen granules and smooth (or partly smooth) vesicles. Presumably, much of the label was added onto growing glycogen granules, in accord with the biochemical view that glycogen may serve as substrate for further glycogen synthesis. The few silver grains located far from glycogen granules-15% at the 15 min interval in vivo-approximated smooth (or partly smooth) vesicles of endoplasmic reticulum. This observation raised the possibility that smooth membranes play a role in g...Continue Reading


Jul 1, 1974·The American Journal of Anatomy·M B Babcock, R R Cardell
Aug 11, 1975·The American Journal of Anatomy·M B Babcock, R R Cardell
Feb 1, 1990·Journal of Electron Microscopy Technique·R R Cardell, E L Cardell
Jan 1, 1973·Histochemie. Histochemistry. Histochimie·L A Lindberg
May 18, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·I L ChenH S Lin
Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·Y J Le Beux
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·N CorvajaO Pompeiano
Oct 3, 1977·Histochemistry·S Benchimol, M Cantin
Jan 1, 1969·Virchows Archiv. A: Pathology. Pathologische Anatomie·R S Sohal, G E Burch
Feb 1, 1976·International Journal for Parasitology·Z Swiderski, J S Mackiewicz
Mar 1, 1968·The Journal of Cell Biology·P R Garant
Oct 1, 1968·The Journal of Cell Biology·R W Young, B Droz
Apr 1, 1969·The Journal of Cell Biology·M M SalpeterE E Salpeter
Oct 1, 1970·The Journal of Cell Biology·M M Magalhães, A Coimbra
May 1, 1973·The Journal of Obstetrics and Gynaecology of the British Commonwealth·E M ArmstrongW R Chatfield
May 1, 1974·The Journal of Obstetrics and Gynaecology of the British Commonwealth·I A MoreD McSeveney
Nov 6, 2002·FEBS Letters·Josep M Fernández-NovellJoan J Guinovart
Feb 1, 1972·Journal of Cellular Physiology·C A Benzo, G De la Haba
Jan 1, 1972·Tissue & Cell·J P Green, M R Neff
Nov 1, 1969·Journal of Ultrastructure Research·E Pannese
Jun 1, 1968·General and Comparative Endocrinology·R S Piezzi, M H Burgos
Jul 1, 1970·Journal of Ultrastructure Research·M M Magalhães, M C Magalhães
Apr 1, 1971·Journal of Ultrastructure Research·S Karasaki
Jul 1, 1967·Journal of Ultrastructure Research·W J Dougherty, M M Lee


Jun 1, 1961·The Journal of Biophysical and Biochemical Cytology·D J LUCK
Nov 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J R TROTT
Feb 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J P REVEL
Feb 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J MOSES
Jun 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B A YOUNG, B M KOPRIWA
Jan 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·J MORDOHC R KRISMAN
May 1, 1955·Stain Technology·P S WOODS, A W POLLISTER
Dec 1, 1961·The Journal of Biophysical and Biochemical Cytology·M J KARNOVSKY

Related Concepts

Glucose, (beta-D)-Isomer
Electron Microscopy
Endoplasmic Reticulum

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.