SIVmac Gag p27 capsid protein gene expression in potato

Protein Expression and Purification
Tae-Geum KimW H R Langridge

Abstract

A cDNA encoding the Simian immunodeficiency virus type (SIV(mac)) Gag capsid protein was introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods. The gag gene was detected in the genomic DNA of transformed leaf tissues by PCR DNA amplification. Immunoblot analysis of transformed potato plant extracts with anti-Gag monoclonal antibody showed that biologically active Gag protein was synthesized in transformed tuber tissues. Based on ELISA results, recombinant Gag protein made up 0.006-0.014% of total soluble tuber protein. The synthesis of SIV Gag in transformed potato tubers opens the way for development of Gag-based edible plant vaccines for protection against SIV and potentially HIV-1 infection.

References

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Citations

May 10, 2005·Immunology and Cell Biology·Diane E WebsterSteve L Wesselingh
Dec 9, 2009·Biologicals : Journal of the International Association of Biological Standardization·Hua YanMingchun Ji

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