SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradation.

BMC Biotechnology
Marinko Sremac, Elizabeth S Stuart

Abstract

Recombinant gas vesicles (r-GV) from Halobacterium sp. strain SD109 expressing cassettes with different SIVsm inserts, have potential utility as an effective antigen display system for immunogen testing in vivo and for initial epitope assessments in vitro. Previous mouse model studies demonstrated immunization with r-GV expressing selected exogenous sequences elicited a prolonged immune response. Here we tested segments from three SIVsm genes (tat, rev, and nef) each surface displayed by r-GV. As with HIV, for SIVsm the proteins encoded by tat, rev and nef respectively serve critical and diverse functions: effects on efficient viral RNA polymerase II transcription, regulation of viral gene expression and effects on specific signaling functions through the assembly of multiprotein complexes. Humoral responses to r-GVTat, Rev or Nef1 elicited in vivo, associated changes in selected cell cytokine production following r-GV internalization, and the capacity of J774A.1 macrophage cells to degrade these internalized display/delivery particles in vitro were examined. The in vivo studies involving r-GV immunizations and in vitro studies of r-GV uptake by J774A.1 macrophages demonstrated: (i) tests for antibody isotypes in immunized mice...Continue Reading

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Citations

Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joshua P RamsayGeorge P C Salmond
Dec 24, 2013·BMC Biotechnology·Shiladitya DasSarmaBarbara Smith
Sep 10, 2015·Vaccines·Shiladitya DasSarma, Priya DasSarma
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Jan 11, 2017·Molecular Pharmaceutics·Abhay U AndarShiladitya DasSarma

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Methods Mentioned

BETA
fluorescence microscopy
ELISA

Software Mentioned

VersaMax

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