Six-year molecular analysis of Burkholderia cepacia complex isolates among cystic fibrosis patients at a referral center for lung transplantation.

Journal of Clinical Microbiology
D G HeathP Gilligan

Abstract

Over a 6-year period, Burkholderia cepacia complex species were isolated from cystic fibrosis (CF) patients receiving care at The University of North Carolina Hospitals (clinic CF patients) and from those referred from other treatment centers. Fifty-six isolates collected from 30 referred patients and 26 clinic CF patients were characterized by pulsed-field gel electrophoresis (PFGE) and were assayed by PCR to detect the cable pilin gene, cblA. PFGE results indicated that six separate clusters (clusters A to F) were present among the 56 isolates and that three clusters (clusters A, B, and E) consisted only of isolates from referred patients infected with B. cepacia complex isolates prior to referral. However, one cluster (cluster C) consisted of isolates from four CF patients, and hospital records indicate that this cluster began with an isolate that came from a referred patient and that spread to three clinic CF patients. Cluster D consisted of two isolates from clinic CF patients, and hospitalization records are consistent with nosocomial, patient-to-patient spread. cblA was present in only 4 of the 56 isolates and included isolates in cluster E from the referred patients. Our results indicate a lack of spread of a previously...Continue Reading

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Citations

Aug 3, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M J Medina-PascualJ A Saéz-Nieto
Jan 17, 2004·Clinical Microbiology Reviews·Lisa Saiman, Jane Siegel
Sep 6, 2003·Journal of Clinical Microbiology·Melissa B Miller, Peter H Gilligan
Jan 13, 2006·BMC Infectious Diseases·Antonietta LambiaseFabio Rossano
May 27, 2014·Clinics in Laboratory Medicine·Peter H Gilligan
May 19, 2007·Diagnostic Microbiology and Infectious Disease·Robert SlingerShawn D Aaron
May 1, 2007·Clinics in Chest Medicine·Hilary J Goldberg, Aaron Deykin
Jan 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S DuaR M Aris
May 15, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Z V VasiljevicB Jovcic
Apr 6, 2004·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Rana M NasserWassim Y Almawi
May 9, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Richard W DoucePaul Cardenas Aldaz
Jul 11, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Adam M BresslerChristopher W Woods
Mar 21, 2006·Pediatric Pulmonology·Marianne S MuhlebachMargaret W Leigh
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·Vincent RamissePhilippe Normand

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