Size and Content of the Sex-Determining Region of the Y Chromosome in Dioecious Mercurialis annua , a Plant with Homomorphic Sex Chromosomes

Genes
Paris VeltsosJohn R Pannell

Abstract

Dioecious plants vary in whether their sex chromosomes are heteromorphic or homomorphic, but even homomorphic sex chromosomes may show divergence between homologues in the non-recombining, sex-determining region (SDR). Very little is known about the SDR of these species, which might represent particularly early stages of sex-chromosome evolution. Here, we assess the size and content of the SDR of the diploid dioecious herb Mercurialis annua, a species with homomorphic sex chromosomes and mild Y-chromosome degeneration. We used RNA sequencing (RNAseq) to identify new Y-linked markers for M. annua. Twelve of 24 transcripts showing male-specific expression in a previous experiment could be amplified by polymerase chain reaction (PCR) only from males, and are thus likely to be Y-linked. Analysis of genome-capture data from multiple populations of M. annua pointed to an additional six male-limited (and thus Y-linked) sequences. We used these markers to identify and sequence 17 sex-linked bacterial artificial chromosomes (BACs), which form 11 groups of non-overlapping sequences, covering a total sequence length of about 1.5 Mb. Content analysis of this region suggests that it is enriched for repeats, has low gene density, and contain...Continue Reading

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Citations

Jun 22, 2019·The New Phytologist·Deborah Charlesworth
Sep 11, 2019·Journal of Experimental Botany·Janardan KhadkaAvi Golan-Goldhirsh
Jul 23, 2018·Annals of Botany·Roman HobzaBohuslav Janousek
May 2, 2020·The New Phytologist·Simona BaránkováSònia Garcia
Oct 25, 2020·Journal of Evolutionary Biology·Guillaume G Cossard, John R Pannell
Jan 21, 2021·Current Biology : CB·Guillaume G CossardJohn R Pannell

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Methods Mentioned

BETA
PCR
RNAseq

Software Mentioned

Geneious
SMRTpipe
RepeatMasker
Augustus
BLAT

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