Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells

Cancer Nanotechnology
Pallavi VedantamT R Jeremy Tzeng

Abstract

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

References

Mar 1, 1997·British Journal of Urology·R PaulW B Isaacs
Oct 29, 2004·Physics in Medicine and Biology·James F HainfeldHenry M Smilowitz
Mar 28, 2006·Biochimica Et Biophysica Acta·Nathan Sharon
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tommy CedervallSara Linse
Oct 30, 2007·Small·Yu PanWilli Jahnen-Dechent
May 6, 2008·Cancer Biotherapy & Radiopharmaceuticals·Joerg LehmannSally J Denardo
Jun 12, 2008·Clinical and Investigative Medicine. Médecine Clinique Et Experimentale·Xiaojing ZhangWilson Roa
Jul 26, 2008·Nature Nanotechnology·Wen JiangWarren C W Chan
Dec 17, 2008·Nanomedicine : Nanotechnology, Biology, and Medicine·Marina A DobrovolskaiaScott E McNeil
Feb 12, 2009·ACS Nano·Paola NativoMathias Brust
Aug 25, 2011·ACS Nano·Martin LundqvistKenneth Dawson
Jan 13, 2012·World Journal of Urology·Eric A Klein, Ian M Thompson
Jan 1, 2008·Nanotechnology, Science and Applications·Po C ChenAdegboyega K Oyelere

❮ Previous
Next ❯

Citations

Mar 4, 2020·International Journal of Molecular Sciences·Amlan ChakrabortyMagdalena Plebanski
Jun 3, 2020·International Journal of Toxicology·Gamze Tilbe SenBelma Kocer-Gumusel
Sep 14, 2019·International Journal of Molecular Sciences·Elham ShahhoseiniMoshi Geso
Sep 7, 2017·Bioconjugate Chemistry·Amlan ChakrabortyMagdalena Plebanski

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
dynamic light scattering

Software Mentioned

Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved