Size-dependent oxygenation and energy status in multicellular tumor spheroids

Advances in Experimental Medicine and Biology
S WalentaW Mueller-Klieser

Abstract

To evaluate interrelationships among the oxygenation, the energy status, and the development of necrosis in tumor microregions, oxygen tensions were measured with microelectrodes, and ATP distributions were determined with a quantitative imaging technique using multicellular spheroids as in vitro tumor models. The results obtained show a positive correlation between central oxygen tensions and ATP concentrations in spheroids. During spheroid growth, both quantities decrease from rather high values to recordings close to or at the background level within similar ranges of spheroid size. Since the emergence of central necroses precedes this drop in energy-rich phosphate, the data may suggest that energy metabolism is not directly involved in the development of necrosis in the spheroids investigated.

Citations

Feb 17, 2009·Journal of Neuro-oncology·Julie G WalkerJohn F de Groot
Jun 16, 2017·Journal of Cellular Physiology·Eddy-Tim VerjansLiliane Schoofs
Dec 31, 1997·The American Journal of Physiology·L A Kunz-SchughartJ P Freyer
May 18, 2020·International Journal of Molecular Sciences·Koichi FujisawaIsao Sakaida

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