Size-dependent passage of liposome nanocarriers with preserved posttransport integrity across the middle-inner ear barriers in rats

Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology
Jing ZouPaavo K J Kinnunen

Abstract

The goal of this study was to evaluate the impact of liposome nanocarrier size on the efficacy of its transport across the middle-inner ear barriers. The dynamic distribution of liposome nanocarriers encapsulating gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid (LPS+Gd-DOTA) of sizes 95, 130, and 240 nm were observed with a 4.7 T magnetic resonance machine after transtympanic injection in Wistar rats. Histology was performed with confocal microscopy using TRITC conjugated LPS+Gd-DOTA. The integrity of the LPS+Gd-DOTA after transportation was evaluated using cryo-transmission electron microscopy (Cryo-TEM). Size-dependent transport of the LPS+Gd-DOTA across the middle-inner ear barriers was shown using magnetic resonance imaging, which indicated that the 95-nm nanocarrier showed the significantly highest transport percentage, that the 130-nm nanocarrier showed moderate transport, and that the 240 nm nanocarrier showed the lowest transport. Histologic examinations showed that the LPS+Gd-DOTA were distributed in the epithelial cells of the utricle, capillaries of the spiral ligament, and the spiral ganglion cells. LPS+Gd-DOTA remained intact in the perilymph after transportation. The nanocarrier delivery strategy used in this...Continue Reading

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Citations

Jul 11, 2013·Nanomedicine·Christian Oliver PritzRudolf Glueckert
Feb 11, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Naila El KechaiAmélie Bochot
Dec 4, 2014·Journal of Nanobiotechnology·Jing ZouIlmari Pyykkö
Jan 7, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Sergio Martín-SaldañaJulio San Román
Aug 12, 2015·International Journal of Pharmaceutics·Naila El KechaiAmélie Bochot
Aug 1, 2014·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Shaulnie MohanMichael Anne Gratton
Nov 14, 2017·BioMed Research International·Dong-Kee Kim
Nov 30, 2019·Frontiers in Cellular Neuroscience·Christopher RathnamKelvin Kwan
May 4, 2017·Drug Delivery and Translational Research·Umberto M MusazziFrancesco Cilurzo
Nov 8, 2020·International Journal of Pharmaceutics·Céline JaudoinAmélie Bochot

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