Size distribution of pressure-decomposed casein micelles studied by dynamic light scattering and AFM.

European Biophysics Journal : EBJ
R GebhardtU Kulozik

Abstract

Reversible and irreversible states of pressure-dissociated casein micelles were studied by in situ light scattering techniques and ex situ atomic force microscopy. AFM experiments performed at ambient pressure reveal heterogeneities across the micelle, suggesting a sub-structure on a 20 nm scale. At pressures between 50 and 250 MPa, the native micelles disintegrate into small fragments on the scale of the observed sub-structure. At pressures above 300 MPa the micelles fully decompose into their monomeric constituents. After pressure release two discrete populations of casein aggregates are observed, depending on the applied initial pressure: Between 160 and 240 MPa stable micelles with diameters near 100 nm without detectable sub-structures are formed. Casein micelles exposed to pressures above 280 MPa re-associate at ambient pressure yielding mini-micelles with diameters near 25 nm. The implications concerning structural models are discussed.

References

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Citations

Apr 5, 2013·The Journal of Dairy Research·Saeed Rahimi YazdiMilena Corredig
Apr 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroko Nomura, Kenji Katayama
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Dec 12, 2007·Macromolecular Bioscience·Ronald GebhardtPeter Müller-Buschbaum
Oct 14, 2010·Chemical Communications : Chem Comm·Andrew J Jackson, Duncan J McGillivray
Oct 21, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·R GebhardtU Kulozik
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·E MetwalliP Müller-Buschbaum
May 3, 2021·Advances in Colloid and Interface Science·Chuan-He Tang
Mar 6, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Mustapha OuanezarAntoine Bouchoux
Feb 23, 2011·The Journal of Physical Chemistry. B·Ronald GebhardtWolfgang Doster

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