Size-exclusion chromatography of plasma proteins with high molecular masses

Journal of Chromatography. a
D JosićL Britsch

Abstract

Two different hydrophilic materials with large pores, Superose 6 and Fractogel EMD BioSec (S), which are designed for size-exclusion chromatography (SEC) of plasma proteins with high molecular masses, are tested for their performance on a preparative scale. The model mixtures are preparations of the clotting factors VIII (FVIII) and IX (FIX). A combination of a Fractogel EMD BioSec (S) column and a Superose 6 column has proved to be particularly effective for separations in a wide molecular size range, from several millions down to about 20,000. Superose 6 showed good results on a small scale as well as on a large scale, even in the molecular mass range over 1,000,000. However, recovery of FVIII clotting activity was less than 70% with this material and therefore not satisfactory. Fractogel did not perform well in terms of separation on a small scale. However, in the case of biopolymers with high molecular masses, separation was improved by using larger columns. With Fractogel, recovery of activity of the two clotting factors FVIII and FIX was satisfactory, above 80%. On a large scale, the active fraction in the clotting factor concentrate was successfully separated from the non-active fraction with either size exclusion (SE) m...Continue Reading

References

Mar 1, 1991·Biological Chemistry Hoppe-Seyler·D JosićJ Reusch
Jan 1, 1989·Haemostasis·R J WagenvoordH C Hemker
Jun 21, 1986·Lancet·T W BarrowcliffeD P Thomas
Mar 1, 1988·Trends in Biochemical Sciences·K Titani, K A Walsh
Jul 21, 1995·Journal of Chromatography. B, Biomedical Applications·L HofferD Josic
Dec 9, 1994·Journal of Chromatography. B, Biomedical Applications·D JosicA Strancar
May 30, 1994·FEBS Letters·M H WernerR J Fisher
Jul 4, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·D JosićA Strancar
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·K Löster, D Josić

❮ Previous
Next ❯

Citations

Sep 17, 2004·Bioprocess and Biosystems Engineering·F MoureM Díaz
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Djuro JosicAndrea Buchacher
May 31, 2001·Haemophilia : the Official Journal of the World Federation of Hemophilia·D JosicA Buchacher
Sep 29, 2012·Se pu = Chinese journal of chromatography·Limei KangZhiguo Su
Sep 26, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Cátia SalvadorA Teresa Caldeira
Apr 6, 2006·Proteomics·Djuro JosicDouglas C Hixson
Oct 8, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S J Yang, S J Mao
Sep 10, 1999·Journal of Chromatography. a·K PockD Josic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.