Size-Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures

Global Challenges
Xi ChenYuping Shan

Abstract

DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force-tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s-1. Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single-molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin-mediated endocytosis.

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Citations

Sep 26, 2020·ACS Applied Materials & Interfaces·Tao ZhangYunfeng Lin

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Methods Mentioned

BETA
atomic force microscopy
electrophoresis
AFM
fluorescence imaging
fluorescence
confocal microscopy

Software Mentioned

LabVIEW
Andorra
MATLAB

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