Nov 20, 2018

Size-selected silver nanoparticles for MALDI-TOF mass spectrometry of amyloid-beta peptides

Nanoscale
Feng DingJianliang Shen

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is one of the most efficient mass spectrometric techniques for the analysis of high-molecular-weight compounds with superior selectivity and sensitivity. Common MALDI matrices are low molecular weight (LMW) organics and will therefore produce a large amount of matrix-related ion peaks, which limits the use of MALDI-MS for the detection of LMW molecules. A major breakthrough of this limitation was made by the introduction of surface assisted desorption/ionization techniques, with graphite particles firstly as the matrix, followed by expansion into other types of nanoparticles or nanostructures. However, previous studies failed to address well the optimum size and concentration of Ag NPs to be used as the MALDI matrix. In this study, to explore and compare the efficiency of different sized silver nanoparticles (Ag NPs) as the MALDI matrix for the detection of LMW molecules, three different sized Ag NPs (2.8 ± 1.0, 12.8 ± 3.2 and 44.2 ± 5.0 nm) have been successfully developed as the MALDI time-of-flight MS (MALDI-TOF MS) matrix and amyloid-beta (Aβ) peptides, crucially involved in Alzheimer's disease and a variety of cancers, were chosen as an example of LMW...Continue Reading

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Mentioned in this Paper

Study
Size
APP protein, human
Natural graphite
TOF MS (Time of Flight Mass Spectrometry)
Extracellular Matrix
Neoplasms
Ions
Alzheimer's Disease
Low Molecular Weight Kininogen Binding

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