Sizing of Single Particles at the Orifice of a Nanopipette

ACS Sensors
Yang LiuLanqun Mao

Abstract

Developing new methods and techniques for size analysis of particles in solution is highly desirable not only for industrial screening of particles but also for single biological entity analysis (e.g., single cells or single vesicles). Herein, we report a new technique for sizing of single particles in solution with a nanopipette. The rationale is essentially based on ion-current blockage when the particles are approaching to the proximity of nanopipette orifice. By rationally controlling the geometry of nanopipette and the applied potential, the spike-type ion current transient generated from the motion of particles in the process of "collision and departure" is employed for sizing of single particles. The results show that both the relative ion-current change (△I/I0) and the dwell time (△t) of spike-type transient are dependent on particle size. Differently, △t is also related to external applied voltage. Statistical analysis shows that △I/I0 is proportional to particle diameter, and this linear relationship is further understood by finite-element simulations. This study not only provides a new principle for sizing of single particles in solution, but also is helpful to understand the motion of particle near the orifice of na...Continue Reading

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Citations

Sep 9, 2020·Chemical Communications : Chem Comm·Jinzheng ZhangFan Xia
Jan 28, 2021·Analytical Chemistry·Tienan GaoLanqun Mao
Feb 24, 2021·ACS Sensors·Lanqun Mao
Jun 18, 2021·ACS Sensors·Cong XuLanqun Mao
Aug 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Silan BaiLishi Wang
Sep 15, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Zhan WangZhi-Yuan Gu

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