Abstract
Using arbitrary primed-PCR (AP-PCR), we have identified a novel genetic alteration located at chromosome 11q23.2 and this genetic alteration was common in 38% of the human Wilms tumour samples analysed. Further characterisation by cloning and sequencing of this genomic region revealed that it represents a part of an uncharacterised gene. We have named this gene as Sporadic Kidney Cancer Gene-1 (SKCG-1). Using fluorescence in situ hybridisation (FISH) approach, we established its localisation on the chromosome 11q23.2. Northern analysis revealed the transcript size of SKCG-1 of 2.09 kb and this was further confirmed by full-length cDNA sequence. Sequence analysis revealed an active translation start site (ATG sequence), a polyadenylation signal sequence (AATAAA), and an open reading frame (ORF) encoding a peptide of 124 amino acids in the cDNA sequence of SKCG-1. Analysis of genomic sequence of SKCG-1 revealed a promoter region containing TATA box located at -13 bp upstream of transcription start site. The AP-PCR, SCAR, and Southern blot analyses indicated genomic loss of SKCG-1 in Wilms tumours. The transcript of SKCG-1 was abundantly present in brain, kidney, liver, testis, salivary gland, foetal brain, foetal liver, whereas r...Continue Reading
References
Feb 9, 1990·Cell·K M CallW H Lewis
Feb 22, 1990·Nature·M GesslerG A Bruns
Dec 1, 1988·Human Genetics·M MannensP L Pearson
Mar 1, 1982·British Journal of Haematology·T PapayannopoulouT Maniatis
Jan 1, 1981·Human Genetics·E Matsunaga
Nov 1, 1994·Molecular and Cellular Biology·M A VellecaJ P Merlie
Jun 10, 1995·Genomics·P RadiceM A Pierotti
Jan 1, 1993·Methods in Enzymology·M A Frohman
Mar 1, 1995·Genomics·T StokkeL Deaven
May 1, 1994·Nature Genetics·N BardeesyJ Pelletier
Jan 1, 1994·Cytogenetics and Cell Genetics·U Francke
May 20, 1996·FEBS Letters·B OlivèsP Bailly
Aug 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Kozak
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S StilgenbauerH Döhner
Jun 27, 1998·The Journal of Biological Chemistry·K TakedaK Miyazono
Jan 11, 2000·Nucleic Acids Research·V A ErdmannJ Barciszewski
Feb 13, 2001·The American Journal of Pathology·S HingK Pritchard-Jones
May 30, 2001·Gene·K P Singh, D Roy
Oct 12, 2001·Gene·V ScharnhorstA G Jochemsen
Citations
Apr 7, 2007·Current Opinion in Oncology·Sharon M Castellino, Thomas W McLean
Sep 21, 2010·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Christopher BlackmoreAru Narendran
Nov 30, 2006·Current Opinion in Oncology