SKCG-1: a new candidate growth regulatory gene at chromosome 11q23.2 in human sporadic Wilms tumours.

British Journal of Cancer
Kamaleshwar P Singh, Deodutta Roy

Abstract

Using arbitrary primed-PCR (AP-PCR), we have identified a novel genetic alteration located at chromosome 11q23.2 and this genetic alteration was common in 38% of the human Wilms tumour samples analysed. Further characterisation by cloning and sequencing of this genomic region revealed that it represents a part of an uncharacterised gene. We have named this gene as Sporadic Kidney Cancer Gene-1 (SKCG-1). Using fluorescence in situ hybridisation (FISH) approach, we established its localisation on the chromosome 11q23.2. Northern analysis revealed the transcript size of SKCG-1 of 2.09 kb and this was further confirmed by full-length cDNA sequence. Sequence analysis revealed an active translation start site (ATG sequence), a polyadenylation signal sequence (AATAAA), and an open reading frame (ORF) encoding a peptide of 124 amino acids in the cDNA sequence of SKCG-1. Analysis of genomic sequence of SKCG-1 revealed a promoter region containing TATA box located at -13 bp upstream of transcription start site. The AP-PCR, SCAR, and Southern blot analyses indicated genomic loss of SKCG-1 in Wilms tumours. The transcript of SKCG-1 was abundantly present in brain, kidney, liver, testis, salivary gland, foetal brain, foetal liver, whereas r...Continue Reading

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Citations

Apr 7, 2007·Current Opinion in Oncology·Sharon M Castellino, Thomas W McLean
Sep 21, 2010·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Christopher BlackmoreAru Narendran
Nov 30, 2006·Current Opinion in Oncology

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Datasets Mentioned

BETA
AP003027
BF002848
AF
525402.1

Methods Mentioned

BETA
electrophoresis
PCR
X-ray
in vitro transcription

Software Mentioned

EMBL bioinformatic harvester Website
BLASTx
SOSUI
BLAST
BLASTn

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