Skeletal cell differentiation is enhanced by atmospheric dielectric barrier discharge plasma treatment

PloS One
Marla J SteinbeckTheresa A Freeman

Abstract

Enhancing chondrogenic and osteogenic differentiation is of paramount importance in providing effective regenerative therapies and improving the rate of fracture healing. This study investigated the potential of non-thermal atmospheric dielectric barrier discharge plasma (NT-plasma) to enhance chondrocyte and osteoblast proliferation and differentiation. Although the exact mechanism by which NT-plasma interacts with cells is undefined, it is known that during treatment the atmosphere is ionized generating extracellular reactive oxygen and nitrogen species (ROS and RNS) and an electric field. Appropriate NT-plasma conditions were determined using lactate-dehydrogenase release, flow cytometric live/dead assay, flow cytometric cell cycle analysis, and Western blots to evaluate DNA damage and mitochondrial integrity. We observed that specific NT-plasma conditions were required to prevent cell death, and that loss of pre-osteoblastic cell viability was dependent on intracellular ROS and RNS production. To further investigate the involvement of intracellular ROS, fluorescent intracellular dyes Mitosox (superoxide) and dihydrorhodamine (peroxide) were used to assess onset and duration after NT-plasma treatment. Both intracellular supe...Continue Reading

References

Jan 1, 1982·Lasers in Surgery and Medicine·J L GloverR J Plunkett
Apr 1, 1995·Free Radical Biology & Medicine·R H Burdon
Aug 12, 1998·Journal of Cellular Physiology·M J SteinbeckM J Karnovsky
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·L ZhangH Fu
Oct 13, 2000·The Journal of Biological Chemistry·K SayamaK Hashimoto
Jan 12, 2001·Molecular and Cellular Biology·A H KimM V Chao
Apr 25, 2001·Journal of Cellular Physiology·A J Gow, H Ischiropoulos
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nobuhiro KamiyaHideto Watanabe
Sep 27, 2003·Acta Physiologica Scandinavica·I N MungrueM Husain
Nov 5, 2003·The Biochemical Journal·Lee-Anne StantonFrank Beier
Nov 5, 2003·Developmental Biology·Lee-Anne StantonFrank Beier
Jan 15, 2004·Gastrointestinal Endoscopy·John J Vargo
Sep 15, 2004·The Journal of Biological Chemistry·Miklós Geiszt, Thomas L Leto
Jan 5, 2005·Biochemical and Biophysical Research Communications·Kazunori KashiwaseKinya Otsu
May 3, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Carole NiccoFrédéric Batteux
May 27, 2005·Nitric Oxide : Biology and Chemistry·Anatoly B ShekhterAnatoly F Vanin
Nov 29, 2005·Cell Death and Differentiation·S AkterinA Cedazo-Minguez
Feb 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ching-Chyuan Hsieh, John Papaconstantinou
May 9, 2006·Antioxidants & Redox Signaling·James R Stone, Suping Yang
May 29, 2007·The International Journal of Biochemistry & Cell Biology·Shazib Pervaiz, Marie-Veronique Clement
Sep 22, 2007·Annual Review of Pharmacology and Toxicology·Kohsuke TakedaHidenori Ichijo
Dec 7, 2007·Annals of the New York Academy of Sciences·Jian-Su ShaoDwight A Towler
Jan 22, 2008·Biochimica Et Biophysica Acta·Atsushi Matsuzawa, Hidenori Ichijo
Apr 19, 2008·Nature Chemical Biology·Christine C Winterbourn
May 6, 2008·Plastic and Reconstructive Surgery·Ines C LinRichard E Kirschner
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jose C JuarezFernando Doñate
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Jan 24, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R CastagnaG Valacchi
Apr 29, 2009·International Journal of Radiation Biology·Chih-Chiang WengBo-Wen Hsu
Jun 27, 2009·Immunopharmacology and Immunotoxicology·Rizwan AhmadHaseeb Ahsan
Dec 17, 2009·Annals of Biomedical Engineering·Sameer KalghatgiAlisa Morss Clyne
Jan 7, 2010·Biochemistry·Henry Jay FormanFulvio Ursini
Feb 19, 2010·Journal of Photochemistry and Photobiology. B, Biology·Toshihiro KushibikiKunio Awazu
Feb 19, 2010·Experimental & Molecular Medicine·Ae-Ri JiYoung Min Choi
Jul 6, 2010·TheScientificWorldJournal·Tiziana ParasassiFulvio Ursini
Jul 24, 2010·Antioxidants & Redox Signaling·Michel RigouletAnne Devin
Oct 30, 2010·The Biochemical Journal·Chandi C MandalNandini Ghosh-Choudhury

❮ Previous
Next ❯

Citations

Aug 8, 2014·Tissue Engineering. Part a·Natalie ChernetsTheresa A Freeman
Dec 10, 2014·Biomolecules & Therapeutics·Beate HaertelUlrike Lindequist
Aug 8, 2018·International Journal of Molecular Sciences·Jiao Jiao ZhangDong Kee Jeong
Dec 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ju Kyeong ParkChul-Ho Kim
Aug 10, 2016·PloS One·Amanda LeeShubha Gururaja Rao
Aug 13, 2020·International Journal of Dermatology·Peter C Friedman
Sep 6, 2019·Journal of Physics D: Applied Physics·Marian KhaliliVandana Miller
May 3, 2019·Oxidative Medicine and Cellular Longevity·Ying LiIhn Han
Aug 12, 2016·Journal of Tissue Engineering and Regenerative Medicine·Peter EisenhauerTheresa A Freeman
Mar 7, 2021·Journal of Clinical Medicine·Miguel Mateu-SanzCristina Canal

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
electrophoresis
PCR

Software Mentioned

MACSQuant
Image quant TL

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis