Skeletal growth factors regulate the synthesis of insulin-like growth factor binding protein-5 in bone cell cultures.

The Journal of Biological Chemistry
E Canalis, B Gabbitas

Abstract

Skeletal cells secrete insulin-like growth factors (IGFs) I and II and six known IGF binding proteins (IGFBPs). IGFBP-5 stimulates bone formation, and its synthesis correlates with changes in osteoblast cell growth. We tested the effects of basic fibroblast growth factor (bFGF), transforming growth factor beta 1 (TGF beta 1), and platelet-derived growth factor (PDGF) BB on IGFBP-5 expression in cultures of osteoblast-enriched cells from 22-day-old fetal rat calvariae (Ob cells). Treatment of Ob cells with bFGF, TGF beta 1, and PDGF BB caused a time- and dose-dependent decrease in IGFBP-5 mRNA levels and inhibited IGFBP-5 polypeptide levels in the extracellular matrix. The effects of bFGF, TGF beta 1, and PDGF BB on IGFBP-5 transcripts were independent of cell division and were observed in the presence and absence of hydroxyurea. bFGF, TGF beta 1, and PDGF BB did not modify the decay of IGFBP-5 mRNA in transcriptionally arrested Ob cells, and they inhibited IGFBP-5 heterogeneous nuclear RNA and the rate of IGFBP-5 transcription. In conclusion, bFGF, TGF beta 1, and PDGF BB inhibit IGFBP-5 expression in Ob cells independently of their mitogenic activity and through mechanisms that involve decreased transcription.

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Citations

Jul 1, 1997·Journal of Cellular Biochemistry·B Gabbitas, E Canalis
Oct 16, 1999·Brain Research. Molecular Brain Research·S PeterssonC Lavebratt
Apr 1, 1997·Baillière's Clinical Endocrinology and Metabolism·K W NgD M Findlay
Apr 26, 2008·Endocrine Reviews·Andrea GiustinaErnesto Canalis
Jul 11, 2009·Endocrine Reviews·Claes OhlssonJohan Svensson
Aug 31, 2000·Biochemical and Biophysical Research Communications·Y T HouE M Zimmerman
Apr 12, 2014·Nature Reviews. Cancer·Robert C Baxter
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Jul 28, 2001·European Journal of Gastroenterology & Hepatology·J M ZeehG Gerken
Apr 24, 2018·Endocrine Reviews·Gherardo MazziottiAndrea Giustina
Dec 8, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ioannis ChristodoulouJulia M Polak
Mar 12, 1998·Endocrine Reviews·T A Guise, G R Mundy
Oct 21, 1999·Molecular Genetics and Metabolism·L A WetterauP Cohen

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